Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index

S Hell, G Reiner, C Cremer… - Journal of microscopy, 1993 - Wiley Online Library
… confocal fluorescence microscopy. Provided that immersion liquids of correct refractive index
… In most cases, however, specimens have a index refractive (n2) which is different to that of …

Refractive index sensing of green fluorescent proteins in living cells using fluorescence lifetime imaging microscopy

HJ Van Manen, P Verkuijlen, P Wittendorp… - Biophysical journal, 2008 - cell.com
We show that fluorescence lifetime imaging microscopy (FLIM) of green fluorescent protein (GFP)
molecules in cells can be used to report on the local refractive index of intracellular …

High refractive index substrates for fluorescence microscopy of biological interfaces with high z contrast

CM Ajo-Franklin, L Kam… - Proceedings of the …, 2001 - National Acad Sciences
… angle total internal reflection fluorescence microscopy (VIA-TIRFM) … Thus, distance information
is encoded in fluorescence … For a given refractive index material, the penetration depth …

Effect of refractive index on the fluorescence lifetime of green fluorescent protein

C Tregidgo, JA Levitt, K Suhling - Journal of biomedical optics, 2008 - spiedigitallibrary.org
… GFP fluorescence lifetime is shortened in TIRF microscopy in … the GFP fluorescence lifetime
can sense the refractive index, we … internal reflection fluorescence (TIRF) microscopy. In TIRF …

Refractive index of tissue measured with confocal microscopy

JJJ Dirckx, LC Kuypers… - Journal of biomedical …, 2005 - spiedigitallibrary.org
… invert confocal fluorescence microscope with a … fluorescence light was measured with
wavelengths longer than 510 nm . With these parameters, the axial resolution of the microscope is …

Effective refractive index and lipid content of extracellular vesicles revealed using optical waveguide scattering and fluorescence microscopy

DLM Rupert, M Mapar, GV Shelke, K Norling… - Langmuir, 2018 - ACS Publications
… intensity distribution, effective refractive index, and lipid and … -based scattering and
fluorescence microscopy setup (56) to … imply a higher effective refractive index and therefore a …

Refractiveindex‐induced aberrations in two‐photon confocal fluorescence microscopy

H Jacobsen, P Hänninen, E Soini… - … of Microscopy, 1994 - Wiley Online Library
… of refractive index mismatch on the image quality in two-photon confocal fluorescence
microscopy … When exciting at 740 nm and recording the fluorescence around 460 nm in a glycerol-…

Effect of the specimen refractive index on the imaging of a confocal fluorescence microscope employing high aperture oil immersion lenses

H Jacobsen, SW Hell - Bioimaging, 1995 - Wiley Online Library
… For the growing community of confocal microscopy users it is … the investigations on the
refractive index mismatch effects on … popular fluorophores in confocal fluorescence microscopy. …

Refractive index sensing using fluorescence lifetime imaging (FLIM)

C Jones, K Suhling - Journal of Physics: Conference Series, 2006 - iopscience.iop.org
… Optical microscopy, and in particular fluorescence microscopy, is a … In fluorescence
microscopy, the exciting light can be eliminated from the image by filters so that only fluorescence on …

Time-resolved fluorescence microscopy

K Suhling, PMW French, D Phillips - Photochemical & Photobiological …, 2005 - pubs.rsc.org
refractive index effect on the GFP fluorescence decay cannot be neglected. Using the
fluorescence lifetime of GFP as a refractive index … image the refractive index around specific GFP-…