Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index

S Hell, G Reiner, C Cremer… - Journal of microscopy, 1993 - Wiley Online Library
… of confocal fluorescence microscopes The effect of refractive-index mismatch, as … on the
image acquisition process in confocal fluorescence microscopy is investigated theoretically. The …

Influence of refractive-index mismatch in high-resolution three-dimensional confocal microscopy

A Diaspro, F Federici, M Robello - Applied optics, 2002 - opg.optica.org
… PSF) that are due to refractive-index mismatch between the medium containing the biological
… the refractive-index mismatch in the case of CLSM performed under a fluorescence regime …

Refractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correction

MJ Booth, T Wilson - Journal of biomedical optics, 2001 - spiedigitallibrary.org
… (2−p) excitation fluorescence microscopes are becoming … We have investigated the effects
of refractive index mismatch … 2−p confocal scanning microscope configurations. We have also …

Refractive index mismatch induced intensity and phase variations in fluorescence confocal, multiphoton and 4Pi-microscopy

A Egner, M Schrader, SW Hell - Optics communications, 1998 - Elsevier
We examine the focal shift and the phase when focusing into a refractive index mismatched
sample. We show that, while the phase at the location of the intensity maximum is not a …

A procedure to determine the correct thickness of an object with confocal microscopy in case of refractive index mismatch

LC Kuypers, WF Decraemer, JJJ Dirckx… - … of microscopy, 2005 - Wiley Online Library
fluorescence microscopy. We study this procedure for a specimen that has a higher refractive
index … of a confocal microscope in case of a water–polymer mismatch to reference values …

High refractive index substrates for fluorescence microscopy of biological interfaces with high z contrast

CM Ajo-Franklin, L Kam… - Proceedings of the …, 2001 - National Acad Sciences
fluorescence microscopy is widely used to confine the excitation of a complex fluorescent
By working with high refractive index solid supports, it is possible to confine even further the …

Refractive Index Mismatch Can Misindicate Anomalous Diffusion in Single‐Focus Fluorescence Correlation Spectroscopy

S Lehmann, S Seiffert… - … Chemistry and Physics, 2015 - Wiley Online Library
… Two-focus FCS was performed on a setup based on an inverted fluorescence microscope
of type MicroTime200 (PicoQuant, Berlin, Germany), consisting of a life science microscope

Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens

CJ de Grauw, JM Vroom, HTM van der Voort… - Applied …, 1999 - opg.optica.org
… , and n 1 is the refractive index of the immersion liquid and n 2 that of the sample. More
serious are the effects of the refractive-index mismatch on the resolution and signal intensity …

Refractiveindex‐induced aberrations in two‐photon confocal fluorescence microscopy

H Jacobsen, P Hänninen, E Soini… - … of Microscopy, 1994 - Wiley Online Library
… The effect of refractive index mismatch on the image quality in two-photon confocal fluorescence
microscopy is investigated by experiment and numerical calculations. The results show …

Refractive index of tissue measured with confocal microscopy

JJJ Dirckx, LC Kuypers… - Journal of biomedical …, 2005 - spiedigitallibrary.org
microscope, as there is no refractive index mismatch. A difference between index of refraction
… We used the Zeiss Lsm 410 invert confocal fluorescence microscope with a 1.2 NA water …