… the genome engineering procedure in Pseudomonas putida. On the delivery date of the
designed oligonucleotides, homologous regions are amplified from the Pseudomonas genome …

… genome engineering with this system by deleting ten individual genomic regions in the
platform P… -replication system, we tested these vectors for fast genome engineering of P. …

… -assisted genome engineering) is a recent example for an elegant two-stage chromosomal
integration of different BGCs for expression in various bacteria including P. putida. This …

… of the large-scale deletion of genomic islands (GIs) coupling with promoter engineering
was developed in P. putida KT2440 for constructing the minimal genome cell factories (MGF) …

… The main synthetic biology tools needed to engineer P. putida include a reliable set of …
genome-editing techniques [15]. A few constitutive promoter libraries have been characterized in …

… In this article, we describe tools for using Pseudomonas putida, a Gram-negative soil
bacterium, as a heterologous host for producing natural products. Pseudomonads are known to …

… Another stratagem entails the deletion of ung and dut genes of the P. putida genome so that
the resulting strain has a high percentage of T x U replacements that make DNA vulnerable …

… Recent advances in genetic engineering The meanwhile large toolbox for genetic and
genome engineering eases the construction workflow of reliable P. …

… Building on the concepts outlined above, we advocate the choice of Pseudomonas putida
strains as microbial platforms pre-endowed with metabolic and stress-endurance traits that are …

… A combination strategy of promoter engineering and metabolic engineering for improving
the mcl-PHA yield in Pseudomonas putida KTU. The modified genes are marked in red fonts. (…