Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection
… the genome engineering procedure in Pseudomonas putida. On the delivery date of the
designed oligonucleotides, homologous regions are amplified from the Pseudomonas genome …
designed oligonucleotides, homologous regions are amplified from the Pseudomonas genome …
[HTML][HTML] Synthetic control of plasmid replication enables target-and self-curing of vectors and expedites genome engineering of Pseudomonas putida
… genome engineering with this system by deleting ten individual genomic regions in the
platform P… -replication system, we tested these vectors for fast genome engineering of P. …
platform P… -replication system, we tested these vectors for fast genome engineering of P. …
Engineering of natural product biosynthesis in Pseudomonas putida
A Loeschcke, S Thies - Current opinion in biotechnology, 2020 - Elsevier
… -assisted genome engineering) is a recent example for an elegant two-stage chromosomal
integration of different BGCs for expression in various bacteria including P. putida. This …
integration of different BGCs for expression in various bacteria including P. putida. This …
Enhanced production of polyhydroxyalkanoates in Pseudomonas putida KT2440 by a combination of genome streamlining and promoter engineering
H Liu, Y Chen, Y Zhang, W Zhao, H Guo… - International Journal of …, 2022 - Elsevier
… of the large-scale deletion of genomic islands (GIs) coupling with promoter engineering
was developed in P. putida KT2440 for constructing the minimal genome cell factories (MGF) …
was developed in P. putida KT2440 for constructing the minimal genome cell factories (MGF) …
Genetic tools for reliable gene expression and recombineering in Pseudomonas putida
TB Cook, JM Rand, W Nurani… - Journal of Industrial …, 2018 - academic.oup.com
… The main synthetic biology tools needed to engineer P. putida include a reliable set of …
genome-editing techniques [15]. A few constitutive promoter libraries have been characterized in …
genome-editing techniques [15]. A few constitutive promoter libraries have been characterized in …
Stepwise genetic engineering of Pseudomonas putida enables robust heterologous production of prodigiosin and glidobactin A
TB Cook, TB Jacobson, MV Venkataraman… - … engineering, 2021 - Elsevier
… In this article, we describe tools for using Pseudomonas putida, a Gram-negative soil
bacterium, as a heterologous host for producing natural products. Pseudomonads are known to …
bacterium, as a heterologous host for producing natural products. Pseudomonads are known to …
[HTML][HTML] Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform
E Martínez-García, V de Lorenzo - Current opinion in biotechnology, 2024 - Elsevier
… Another stratagem entails the deletion of ung and dut genes of the P. putida genome so that
the resulting strain has a high percentage of T x U replacements that make DNA vulnerable …
the resulting strain has a high percentage of T x U replacements that make DNA vulnerable …
[HTML][HTML] Industrial biotechnology of Pseudomonas putida: advances and prospects
… Recent advances in genetic engineering The meanwhile large toolbox for genetic and
genome engineering eases the construction workflow of reliable P. …
genome engineering eases the construction workflow of reliable P. …
[HTML][HTML] Genome reduction boosts heterologous gene expression in Pseudomonas putida
S Lieder, PI Nikel, V de Lorenzo, R Takors - Microbial cell factories, 2015 - Springer
… Building on the concepts outlined above, we advocate the choice of Pseudomonas putida
strains as microbial platforms pre-endowed with metabolic and stress-endurance traits that are …
strains as microbial platforms pre-endowed with metabolic and stress-endurance traits that are …
A promoter engineering-based strategy enhances polyhydroxyalkanoate production in Pseudomonas putida KT2440
Y Zhang, H Liu, Y Liu, K Huo, S Wang, R Liu… - International Journal of …, 2021 - Elsevier
… A combination strategy of promoter engineering and metabolic engineering for improving
the mcl-PHA yield in Pseudomonas putida KTU. The modified genes are marked in red fonts. (…
the mcl-PHA yield in Pseudomonas putida KTU. The modified genes are marked in red fonts. (…
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