Background The production of recombinant monoclonal antibodies in mammalian cell
culture is of high priority in research and medical fields. A critical step in this process is the …

Background The demand of monospecific high affinity binding reagents, particularly
monoclonal antibodies, has been steadily increasing over the last years. Enhanced …

The isolation of mammalian cell lines capable of high-yield expression of recombinant
antibodies is typically performed by screening multiple individual clones by limiting dilution …

As a growing number of therapeutic antibodies are developed, robust methods to efficiently
improve the affinity and/or specificity of antibody candidates are needed. Here we describe …

A prerequisite for the use of recombinant antibody technologies starting from hybridomas or
immune repertoires is the reliable cloning of functional immunoglobulin genes. For this …

Bispecific antibodies are artificially engineered monoclonal antibodies (MAbs) that consist of
two distinct binding sites and are capable of binding two different antigens noncovalently …

While today antibodies can be obtained from naive repertoires (Winter et al. 1994, Vaughan
et al. 1996) or libraries of fully synthetic genes (Knappik et al. 2000), very many hybridomas …

Background There is an ever-increasing need of monoclonal antibodies (mAbs) for
biomedical applications and fully human binders are particularly desirable due to their …

Rabbit-derived recombinant antibodies have traditionally been viewed as intractable
molecules due to the presence of a cysteine in position 80 of the VL domain that becomes …

Some hybridoma cell lines are very low producers, difficult to cultivate, or antibody
production is lost upon prolonged culture. In these cases, a recombinant" hybridoma …