ScFv improvement approaches
P Bemani, M Mohammadi… - Protein and Peptide …, 2018 - ingentaconnect.com
The most common recombinant antibody format is the single chain fragment variable (scFv)
which it contains the complete antigen-binding domains of an intact antibody. ScFv …
which it contains the complete antigen-binding domains of an intact antibody. ScFv …
With or without sugar?(A) glycosylation of therapeutic antibodies
Antibodies and antibody-based drugs are currently the fastest-growing class of therapeutics.
Over the last three decades, more than 30 therapeutic monoclonal antibodies and …
Over the last three decades, more than 30 therapeutic monoclonal antibodies and …
Single chain Fab (scFab) fragment
M Hust, T Jostock, C Menzel, B Voedisch, A Mohr… - BMC …, 2007 - Springer
Background The connection of the variable part of the heavy chain (VH) and and the
variable part of the light chain (VL) by a peptide linker to form a consecutive polypeptide …
variable part of the light chain (VL) by a peptide linker to form a consecutive polypeptide …
Strategies and considerations for improving recombinant antibody production and quality in Chinese hamster ovary cells
JH Zhang, LL Shan, F Liang, CY Du… - Frontiers in bioengineering …, 2022 - frontiersin.org
Recombinant antibodies are rapidly developing therapeutic agents; approximately 40 novel
antibody molecules enter clinical trials each year, most of which are produced from Chinese …
antibody molecules enter clinical trials each year, most of which are produced from Chinese …
High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells
Background The demand of monospecific high affinity binding reagents, particularly
monoclonal antibodies, has been steadily increasing over the last years. Enhanced …
monoclonal antibodies, has been steadily increasing over the last years. Enhanced …
Advances in the production and batch reformatting of phage antibody libraries
RH Reader, RG Workman, BC Maddison… - Molecular …, 2019 - Springer
Phage display antibody libraries have proven an invaluable resource for the isolation of
diagnostic and potentially therapeutic antibodies, the latter usually being antibody fragments …
diagnostic and potentially therapeutic antibodies, the latter usually being antibody fragments …
Considerations for the design of antibody-based therapeutics
Antibody-based proteins have become an important class of biologic therapeutics, due in
large part to the stability, specificity, and adaptability of the antibody framework. Indeed …
large part to the stability, specificity, and adaptability of the antibody framework. Indeed …
Antibody engineering
J Maynard, G Georgiou - Annual review of biomedical …, 2000 - annualreviews.org
▪ Abstract Antibodies are unique in their high affinity and specificity for a binding partner, a
quality that has made them one of the most useful molecules for biotechnology and …
quality that has made them one of the most useful molecules for biotechnology and …
Design and generation of humanized single-chain Fv derived from mouse hybridoma for potential targeting application
K Khantasup, W Chantima, C Sangma… - Monoclonal …, 2015 - liebertpub.com
Single-chain variable antibody fragments (scFvs) are attractive candidates for targeted
immunotherapy in several human diseases. In this study, a concise humanization strategy …
immunotherapy in several human diseases. In this study, a concise humanization strategy …
Efficient mAb production in CHO cells with optimized signal peptide, codon, and UTR
M You, Y Yang, C Zhong, F Chen, X Wang, T Jia… - Applied microbiology …, 2018 - Springer
Antibody drugs have been used to treat a number of diseases successfully. Producing
antibodies with high yield and quality is necessary for clinical applications of antibodies. For …
antibodies with high yield and quality is necessary for clinical applications of antibodies. For …