Single-chain variable antibody fragments (scFvs) are attractive candidates for targeted
immunotherapy in several human diseases. In this study, a concise humanization strategy …

Provision of effective monoclonal antibody (mAb) based therapeutics requires careful
combination of expression, selection and screening, in a suitable cell culture platform. The …

The clinical success of mAbs continues to reinforce antibody engineering as an essential
tool for the development of biologics. Research focused on discovering the next generation …

Disease intervention by targeting a critical pathway molecule through a blocking antibody or
interference by therapeutic proteins is currently en vogue. Generation of blocking antibodies …

Cell-free protein synthesis is of increasing interest for the rapid and high-throughput
synthesis of many proteins, in particular also antibody fragments. In this study, we present a …

Generation of recombinant antibody fragments has been advanced by phage display
technology but their broad use in biochemical or analytical applications is often hindered by …

In this work, we have investigated parameters important for the assembly of synthetic genes
encoding antibody fragments. These genes are constructed from a set of overlapping single …

High-throughput generation of antibodies against cellular components is currently a
challenge in proteomics, therapeutic development and other biological applications. It is …

The engineering of therapeutic and clinical candidate monoclonal antibodies and Fc fusion
proteins is becoming more sophisticated at generating molecules that are better suited to the …

Generation of sequence defined antibodies from universal libraries by phage display has
been established over the past three decades as a robust method to cope with the …