[HTML][HTML] Cell-free synthesis meets antibody production: a review

M Stech, S Kubick - Antibodies, 2015 - mdpi.com
Engineered antibodies are key players in therapy, diagnostics and research. In addition to
full size immunoglobulin gamma (IgG) molecules, smaller formats of recombinant …

[HTML][HTML] Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates

M Stech, O Nikolaeva, L Thoring, WFM Stöcklein… - Scientific reports, 2017 - nature.com
Antibodies are indispensable tools for basic research as well as diagnostic and therapeutic
applications. Consequently, the development of alternative manufacturing strategies which …

[HTML][HTML] Bioengineering of antibody fragments: challenges and opportunities

S Pirkalkhoran, WR Grabowska, HH Kashkoli… - Bioengineering, 2023 - mdpi.com
Antibody fragments are used in the clinic as important therapeutic proteins for treatment of
indications where better tissue penetration and less immunogenic molecules are needed …

[HTML][HTML] Expression of recombinant antibodies

A Frenzel, M Hust, T Schirrmann - Frontiers in immunology, 2013 - frontiersin.org
Recombinant antibodies are highly specific detection probes in research, diagnostics, and
have emerged over the last two decades as the fastest growing class of therapeutic proteins …

Microbial platform technology for recombinant antibody fragment production: a review

SK Gupta, P Shukla - Critical reviews in microbiology, 2017 - Taylor & Francis
Recombinant antibody fragments are being used for the last few years as an important
therapeutic protein to cure various critical and life threatening human diseases. Several …

Single-chain antibody fragments: Purification methodologies

LP Malpiedi, CA Diaz, BB Nerli, A Pessoa Jr - Process Biochemistry, 2013 - Elsevier
At present, single-chain variable fragments (scFv) of antibodies are considered one of the
most important tools in human therapies. Wide applications of antibodies are being …

Recombinant antibodies: engineering and production in yeast and bacterial hosts

KJ Jeong, SH Jang, N Velmurugan - Biotechnology journal, 2011 - Wiley Online Library
After the appearance of the first FDA‐approved antibody 25 years ago, antibodies have
become major therapeutic agents in the treatment of many human diseases, including …

[HTML][HTML] Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs

A De Marco - Microbial cell factories, 2015 - Springer
Background Antibodies have been a pillar of basic research, while their relevance in clinical
diagnostics and therapy is constantly growing. Consequently, the production of both …

Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system

M Stech, H Merk, JA Schenk, WFM Stöcklein… - Journal of …, 2013 - Elsevier
Cell-free protein synthesis is of increasing interest for the rapid and high-throughput
synthesis of many proteins, in particular also antibody fragments. In this study, we present a …

A simplified and robust protocol for immunoglobulin expression in Escherichia coli cell‐free protein synthesis systems

Q Cai, JA Hanson, AR Steiner, C Tran… - Biotechnology …, 2015 - Wiley Online Library
Cell‐free protein synthesis (CFPS) systems allow for robust protein expression with easy
manipulation of conditions to improve protein yield and folding. Recent technological …