Background Many applications of CRISPR/Cas9-mediated genome editing require Cas9-
induced non-homologous end joining (NHEJ), which was thought to be error prone …

CRISPR-Cas9–mediated homology-directed DNA repair is the method of choice for precise
gene editing in a wide range of model organisms, including mouse and human. Broad use …

Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …

Genome editing using clustered regularly interspaced short palindromic repeats
(CRISPR)/CRISPR-associated protein 9 (Cas9) predominantly induces non-homologous …

The CRISPR-Cas9 system is a powerful genome-editing tool in which a guide RNA targets
Cas9 to a site in the genome, where the Cas9 nuclease then induces a double-stranded …

Genome editing using CRISPR-Cas9 nucleases is based on the repair of the DNA double-
strand break (DSB). In eukaryotic cells, DSBs are rejoined through homology-directed repair …

Precise genome editing/correction of DNA double-strand breaks (DSBs) induced by
CRISPR-Cas9 by homology-dependent repair (HDR) is limited by the competing error-prone …

Precise genome editing requires the resolution of nuclease-induced DNA double strand
breaks (DSBs) via the homology-directed repair (HDR) pathway. In mammals, this is …

Background Due to post-cleavage residence of the Cas9-sgRNA complex at its target, Cas9-
induced DNA double-strand breaks (DSBs) have to be exposed to engage DSB repair …

Methods to introduce targeted double-strand breaks (DSBs) into DNA enable precise
genome editing by increasing the rate at which externally supplied DNA fragments are …