Recent study shows that the prime editing system fusing the Cas9 nickase and reverse
transcriptase could perform all types of gene modifications, including base substitutions …

Dear Editor, The most advanced prime editor 3 (PE3) system comprises the editor, a fusion
protein of Cas9 H840A nickase and mutant reverse transcriptase (RTase)(hereafter termed …

Prime editor (PE), which is developed by combining Cas9 nickase and an engineered
reverse transcriptase, can mediate all twelve types of base substitutions and small insertions …

Prime editors consisting of Cas9-nickase and reverse transcriptase enable targeted precise
editing of small DNA pieces, including all 12 kinds of base substitutions, insertions and …

Prime editing (PE) is a novel CRISPR-derived genome editing technique facilitating
precision editing without double-stranded DNA breaks. PE, mediated by a Cas9-reverse …

The prime editor is a versatile tool for targeted precise editing to generate point mutations,
small insertions, or small deletions in eukaryotes. However, canonical PE3 system is less …

Large scale genomic aberrations including duplication, deletion, translocation, and other
structural changes are the cause of a subtype of hereditary genetic disorders and contribute …

Originating as a component of prokaryotic adaptive immunity, the type II CRISPR/Cas9
system has been repurposed for targeted genome editing in various organisms. Although …

Prime editing recently emerged as a next-generation approach for precise genome editing.
Here we exploit DNA double-strand break (DSB) repair to develop two strategies that install …

Prime editing enables the installation of virtually any combination of point mutations, small
insertions or small deletions in the DNA of living cells. A prime editing guide RNA (pegRNA) …