[HTML][HTML] Non-homologous end joining and homology directed DNA repair frequency of double-stranded breaks introduced by genome editing reagents
M Zaboikin, T Zaboikina, C Freter, N Srinivasakumar - PloS one, 2017 - journals.plos.org
Genome editing using transcription-activator like effector nucleases or RNA guided
nucleases allows one to precisely engineer desired changes within a given target …
nucleases allows one to precisely engineer desired changes within a given target …
[HTML][HTML] Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing
Y Miyaoka, JR Berman, SB Cooper, SJ Mayerl… - Scientific reports, 2016 - nature.com
Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA
nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However …
nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However …
[图书][B] Detection and quantification of HDR and NHEJ induced by genome editing at endogenous gene loci using droplet digital PCR
Y Miyaoka, SJ Mayerl, AH Chan, BR Conklin - 2018 - Springer
Genome editing holds great promise for experimental biology and potential clinical use. To
successfully utilize genome editing, it is critical to sensitively detect and quantify its …
successfully utilize genome editing, it is critical to sensitively detect and quantify its …
Ligation-assisted homologous recombination enables precise genome editing by deploying both MMEJ and HDR
Z Zhao, P Shang, F Sage, N Geijsen - Nucleic Acids Research, 2022 - academic.oup.com
CRISPR/Cas12a is a single effector nuclease that, like CRISPR/Cas9, has been harnessed
for genome editing based on its ability to generate targeted DNA double strand breaks …
for genome editing based on its ability to generate targeted DNA double strand breaks …
[HTML][HTML] Programmable genome editing tools and their regulation for efficient genome engineering
Targeted genome editing has become a powerful genetic tool for studying gene function or
for modifying genomes by correcting defective genes or introducing genes. A variety of …
for modifying genomes by correcting defective genes or introducing genes. A variety of …
[HTML][HTML] Enhancement of homology-directed repair with chromatin donor templates in cells
G Cruz-Becerra, JT Kadonaga - Elife, 2020 - elifesciences.org
A key challenge in precise genome editing is the low efficiency of homology-directed repair
(HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a …
(HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a …
Control of gene editing by manipulation of DNA repair mechanisms
DNA double-strand breaks (DSBs) are produced intentionally by RNA-guided nucleases to
achieve genome editing through DSB repair. These breaks are repaired by one of two main …
achieve genome editing through DSB repair. These breaks are repaired by one of two main …
[HTML][HTML] Effective control of large deletions after double-strand breaks by homology-directed repair and dsODN insertion
Background After repairing double-strand breaks (DSBs) caused by CRISPR-Cas9
cleavage, genomic damage, such as large deletions, may have pathogenic consequences …
cleavage, genomic damage, such as large deletions, may have pathogenic consequences …
Gene editing using ssODNs with engineered endonucleases
F Chen, SM Pruett-Miller, GD Davis - Chromosomal Mutagenesis, 2015 - Springer
Gene editing using engineered endonucleases, such as zinc finger nucleases (ZFNs),
transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced …
transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced …
Novel fluorescent genome editing reporters for monitoring DNA repair pathway utilization at endonuclease-induced breaks
R Kuhar, KS Gwiazda, O Humbert, T Mandt… - Nucleic acids …, 2014 - academic.oup.com
The creation of a DNA break at a specific locus by a designer endonuclease can be
harnessed to edit a genome. However, DNA breaks may engage one of several competing …
harnessed to edit a genome. However, DNA breaks may engage one of several competing …