CRISPR-Cas technologies have the potential to revolutionize genetic medicine. However,
work is still needed to make this technology clinically efficient for gene correction. A barrier …

With the introduction of precision genome editing using CRISPR–Cas9 technology, we have
entered a new era of genetic engineering and gene therapy. With RNA-guided …

CRISPR-Cas9-mediated genome editing holds great promise for the correction of
pathogenic variants in humans. However, its therapeutic implementation is hampered due to …

As CRISPR-Cas systems advance toward clinical application, it is essential to identify all the
outcomes of gene-editing activity in human cells. Reports highlighting the remarkable …

The CRISPR-Cas9 system is a powerful genome-editing tool that promises application for
gene editing therapies. The Cas9 nuclease is directed to the DNA by a programmable single …

Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

CRISPR-Cas9–mediated homology-directed DNA repair is the method of choice for precise
gene editing in a wide range of model organisms, including mouse and human. Broad use …

Introduction: Targeted genome editing using the CRISPR/Cas9 technology is becoming a
major area of research due to its high potential for the treatment of genetic diseases. Our …

The insertion of precise genetic modifications by genome editing tools such as CRISPR-
Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared …

The CRISPR-Cas9 system has successfully been adapted to edit the genome of various
organisms. However, our ability to predict the editing outcome at specific sites is limited …