Improved genome editing by an engineered CRISPR-Cas12a
CRISPR-Cas12a is an RNA-guided, programmable genome editing enzyme found within
bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single …
bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single …
Heterochromatin delays CRISPR-Cas9 mutagenesis but does not influence the outcome of mutagenic DNA repair
EM Kallimasioti-Pazi, K Thelakkad Chathoth… - PLoS …, 2018 - journals.plos.org
Genome editing occurs in the context of chromatin, which is heterogeneous in structure and
function across the genome. Chromatin heterogeneity is thought to affect genome editing …
function across the genome. Chromatin heterogeneity is thought to affect genome editing …
Methods for optimizing CRISPR-Cas9 genome editing specificity
Advances in the development of delivery, repair, and specificity strategies for the CRISPR-
Cas9 genome engineering toolbox are helping researchers understand gene function with …
Cas9 genome engineering toolbox are helping researchers understand gene function with …
[HTML][HTML] Advance trends in targeting homology-directed repair for accurate gene editing: An inclusive review of small molecules and modified CRISPR-Cas9 systems
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Introduction: Clustered regularly interspaced short palindromic repeat and its associated …
Introduction: Clustered regularly interspaced short palindromic repeat and its associated …
Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair
MS Schubert, B Thommandru, J Woodley, R Turk… - Scientific Reports, 2021 - nature.com
CRISPR–Cas proteins are RNA-guided nucleases used to introduce double-stranded
breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular …
breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular …
Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease
RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic
and clinical research, but the determinants of targeting specificity and the extent of off-target …
and clinical research, but the determinants of targeting specificity and the extent of off-target …
Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining
T Maruyama, SK Dougan, MC Truttmann… - Nature …, 2015 - nature.com
Methods to introduce targeted double-strand breaks (DSBs) into DNA enable precise
genome editing by increasing the rate at which externally supplied DNA fragments are …
genome editing by increasing the rate at which externally supplied DNA fragments are …
Genome editing: the road of CRISPR/Cas9 from bench to clinic
A Eid, MM Mahfouz - Experimental & Molecular Medicine, 2016 - nature.com
Molecular scissors engineered for site-specific modification of the genome hold great
promise for effective functional analyses of genes, genomes and epigenomes and could …
promise for effective functional analyses of genes, genomes and epigenomes and could …
Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression
J Rosenbluh, H Xu, W Harrington, S Gill… - Nature …, 2017 - nature.com
CRISPR-Cas9 provides the means to perform genome editing and facilitates loss-of-function
screens. However, we and others demonstrated that expression of the Cas9 endonuclease …
screens. However, we and others demonstrated that expression of the Cas9 endonuclease …
CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites
R Jayavaradhan, DM Pillis, M Goodman… - Nature …, 2019 - nature.com
Precise genome editing/correction of DNA double-strand breaks (DSBs) induced by
CRISPR-Cas9 by homology-dependent repair (HDR) is limited by the competing error-prone …
CRISPR-Cas9 by homology-dependent repair (HDR) is limited by the competing error-prone …