Cas9 targeting and the CRISPR revolution
R Barrangou - Science, 2014 - science.org
The ability to add, remove, or change DNA sequences is essential to studies that investigate
the genetic underpinning of phenotypic traits. With its unprecedented efficiency and stunning …
the genetic underpinning of phenotypic traits. With its unprecedented efficiency and stunning …
[HTML][HTML] CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
[HTML][HTML] CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation
R Tuladhar, Y Yeu, J Tyler Piazza, Z Tan… - Nature …, 2019 - nature.com
The introduction of insertion-deletions (INDELs) by non-homologous end-joining (NHEJ)
pathway underlies the mechanistic basis of CRISPR-Cas9-directed genome editing …
pathway underlies the mechanistic basis of CRISPR-Cas9-directed genome editing …
CRISPR/Cas9 in genome editing and beyond
The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …
Recent advances in CRISPR‐Cas9 genome editing technology for biological and biomedical investigations
ABSTRACT The Type II CRISPR‐Cas9 system is a simple, efficient, and versatile tool for
targeted genome editing in a wide range of organisms and cell types. It continues to gain …
targeted genome editing in a wide range of organisms and cell types. It continues to gain …
Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining
T Maruyama, SK Dougan, MC Truttmann… - Nature …, 2015 - nature.com
Methods to introduce targeted double-strand breaks (DSBs) into DNA enable precise
genome editing by increasing the rate at which externally supplied DNA fragments are …
genome editing by increasing the rate at which externally supplied DNA fragments are …
[HTML][HTML] Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR–Cas9 and ssODN in human cancer cells
Background Precise genome editing is essential for both basic and translational research.
The recently developed CRISPR/Cas9 system can specifically cleave a designated site of …
The recently developed CRISPR/Cas9 system can specifically cleave a designated site of …
[HTML][HTML] Principles of genetic engineering
TM Lanigan, HC Kopera, TL Saunders - Genes, 2020 - mdpi.com
Genetic engineering is the use of molecular biology technology to modify DNA sequence (s)
in genomes, using a variety of approaches. For example, homologous recombination can be …
in genomes, using a variety of approaches. For example, homologous recombination can be …
Nuclear domain 'knock-in'screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing
J Pinder, J Salsman, G Dellaire - Nucleic acids research, 2015 - academic.oup.com
CRISPR is a genome-editing platform that makes use of the bacterially-derived
endonuclease Cas9 to introduce DNA double-strand breaks at precise locations in the …
endonuclease Cas9 to introduce DNA double-strand breaks at precise locations in the …
[HTML][HTML] Improving the efficiency of CRISPR-Cas12a-based genome editing with site-specific covalent Cas12a-crRNA conjugates
The CRISPR-Cas12a system shows unique features compared with widely used Cas9,
making it an attractive and potentially more precise alternative. However, the adoption of this …
making it an attractive and potentially more precise alternative. However, the adoption of this …