CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the
functionality of genetic elements, creating genetically modified organisms as well as …

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated
protein 9 (Cas9) system, an RNA-guided nuclease for specific genome editing in vivo, has …

The CRISPR-Cas system has undoubtedly revolutionized the genome editing field, enabling
targeted gene disruption, regulation, and recovery in a guide RNA-specific manner. In this …

CRISPR–Cas9 editing shows promise for correcting disease-causing mutations. For
example, in a recent study we used CRISPR-Cas9 for sight restoration in blind rd1 mice by …

Precise gene manipulation by gene editing approaches facilitates the potential to cure
several debilitating genetic disorders. Gene modification stimulated by engineered …

Double-stranded DNA (dsDNA) cleavage by Cas9 is a hallmark of type II CRISPR-Cas
immune systems. Cas9-guide RNA complexes recognize 20-base-pair sequences in DNA …

The CRISPR/Cas9 system is a robust genome editing technology that works in human cells,
animals and plants based on the RNA-programmed DNA cleaving activity of the Cas9 …

Bacterial-derived CRISPR-Cas9 nucleases have become a common tool in genome
engineering. However, the editing efficiency by even the best-crafted Cas9 nucleases varies …

Programmable nucleases, such as Cas9, are used for precise genome editing by homology-
dependent repair (HDR),,. However, HDR efficiency is constrained by competition from other …

CRISPR/Cas genome editing is a simple, cost effective, and highly specific technique for
introducing genetic variations. In mammalian cells, CRISPR/Cas can facilitate non …