CRISPR/Cas9‐Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida
T Aparicio, V de Lorenzo… - Biotechnology …, 2018 - Wiley Online Library
While adoption of single‐stranded DNA recombineering techniques has greatly eased
genetic design of the platform strain Pseudomonas putida KT2440, available methods still …
genetic design of the platform strain Pseudomonas putida KT2440, available methods still …
CRISPR/Cas9‐enhanced ssDNA recombineering for Pseudomonas putida
T Aparicio, V de Lorenzo… - Microbial …, 2019 - Wiley Online Library
Implementation of single‐stranded DNA (ss DNA) recombineering in Pseudomonas putida
has widened the range of genetic manipulations applicable to this biotechnologically …
has widened the range of genetic manipulations applicable to this biotechnologically …
Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection
Pseudomonas species have become reliable platforms for bioproduction due to their
capability to tolerate harsh conditions imposed by large‐scale bioprocesses and their …
capability to tolerate harsh conditions imposed by large‐scale bioprocesses and their …
An expanded CRISPR–Cas9-assisted recombineering toolkit for engineering genetically intractable Pseudomonas aeruginosa isolates
D Pankratz, NO Gomez, A Nielsen, A Mustafayeva… - Nature protocols, 2023 - nature.com
Much of our current understanding of microbiology is based on the application of genetic
engineering procedures. Since their inception (more than 30 years ago), methods based …
engineering procedures. Since their inception (more than 30 years ago), methods based …
Modular (de) construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing
CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …
Coupling the CRISPR/Cas9 system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia coli
ME Pyne, M Moo-Young, DA Chung… - Applied and …, 2015 - Am Soc Microbiol
To date, most genetic engineering approaches coupling the type II Streptococcus pyogenes
clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system to lambda …
clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system to lambda …
Bacterial genetic engineering by means of recombineering for reverse genetics
U Fels, K Gevaert, P Van Damme - Frontiers in Microbiology, 2020 - frontiersin.org
Serving a robust platform for reverse genetics enabling the in vivo study of gene functions
primarily in enterobacteriaceae, recombineering-or recombination-mediated genetic …
primarily in enterobacteriaceae, recombineering-or recombination-mediated genetic …
[HTML][HTML] Fundamental CRISPR-Cas9 tools and current applications in microbial systems
Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized
conventional genetic engineering methods and unprecedentedly facilitated strain …
conventional genetic engineering methods and unprecedentedly facilitated strain …
CRISPR-Cas12a-assisted recombineering in bacteria
MY Yan, HQ Yan, GX Ren, JP Zhao… - Applied and …, 2017 - Am Soc Microbiol
Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a (Cpf1) has
emerged as an effective genome editing tool in many organisms. Here, we developed and …
emerged as an effective genome editing tool in many organisms. Here, we developed and …
CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering
Existing technologies for site-specific integration of kilobase-sized DNA sequences in
bacteria are limited by low efficiency, a reliance on recombination, the need for multiple …
bacteria are limited by low efficiency, a reliance on recombination, the need for multiple …