While adoption of single‐stranded DNA recombineering techniques has greatly eased
genetic design of the platform strain Pseudomonas putida KT2440, available methods still …

Implementation of single‐stranded DNA (ss DNA) recombineering in Pseudomonas putida
has widened the range of genetic manipulations applicable to this biotechnologically …

Pseudomonas species have become reliable platforms for bioproduction due to their
capability to tolerate harsh conditions imposed by large‐scale bioprocesses and their …

Much of our current understanding of microbiology is based on the application of genetic
engineering procedures. Since their inception (more than 30 years ago), methods based …

CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

To date, most genetic engineering approaches coupling the type II Streptococcus pyogenes
clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system to lambda …

Serving a robust platform for reverse genetics enabling the in vivo study of gene functions
primarily in enterobacteriaceae, recombineering-or recombination-mediated genetic …

Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized
conventional genetic engineering methods and unprecedentedly facilitated strain …

Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a (Cpf1) has
emerged as an effective genome editing tool in many organisms. Here, we developed and …

Existing technologies for site-specific integration of kilobase-sized DNA sequences in
bacteria are limited by low efficiency, a reliance on recombination, the need for multiple …