Sample size effects on the assessment of eukaryotic diversity and community structure in aquatic sediments using high-throughput sequencing

FJA Nascimento, D Lallias, HM Bik, S Creer - Scientific reports, 2018 - nature.com
Understanding how biodiversity changes in time and space is vital to assess the effects of
environmental change on benthic ecosystems. Due to the limitations of morphological …

Metabarcoding successfully tracks temporal changes in eukaryotic communities in coastal sediments

IS Salonen, PM Chronopoulou… - FEMS Microbiology …, 2019 - academic.oup.com
Metabarcoding is a method that combines high-throughput DNA sequencing and DNA-
based identification. Previously, this method has been successfully used to target spatial …

Influence of commercial DNA extraction kit choice on prokaryotic community metrics in marine sediment

GA Ramírez, D Graham… - Limnology and …, 2018 - Wiley Online Library
Commercial DNA extraction kits are widely used for cultivation‐free surveys of marine
sediment. However, the consequences of popular extraction‐kit choices for sequence …

Grab what you can—an evaluation of spatial replication to decrease heterogeneity in sediment eDNA metabarcoding

JT Hestetun, A Lanzén, TG Dahlgren - PeerJ, 2021 - peerj.com
Environmental DNA methods such as metabarcoding have been suggested as possible
alternatives or complements to the current practice of morphology-based diversity …

High throughput shotgun sequencing of eRNA reveals taxonomic and derived functional shifts across a benthic productivity gradient

E Broman, S Bonaglia, A Norkko, S Creer… - Molecular …, 2021 - Wiley Online Library
Benthic macrofauna is regularly used in monitoring programmes, however the vast majority
of benthic eukaryotic biodiversity lies mostly in microscopic organisms, such as meiofauna …

DNA extraction replicates improve diversity and compositional dissimilarity in metabarcoding of eukaryotes in marine sediments

A Lanzén, K Lekang, I Jonassen, EM Thompson… - PLoS …, 2017 - journals.plos.org
Human impact on marine benthic communities has traditionally been assessed using visible
morphological traits and has focused on the macrobenthos, whereas the ecologically …

High-throughput sequencing and morphology perform equally well for benthic monitoring of marine ecosystems

F Lejzerowicz, P Esling, L Pillet, TA Wilding, KD Black… - Scientific reports, 2015 - nature.com
Environmental diversity surveys are crucial for the bioassessment of anthropogenic impacts
on marine ecosystems. Traditional benthic monitoring relying on morphotaxonomic …

Evaluating sediment and water sampling methods for the estimation of deep-sea biodiversity using environmental DNA

MI Brandt, F Pradillon, B Trouche, N Henry… - Scientific Reports, 2021 - nature.com
Despite representing one of the largest biomes on earth, biodiversity of the deep seafloor is
still poorly known. Environmental DNA metabarcoding offers prospects for fast inventories …

Depth shapes α‐and β‐diversities of microbial eukaryotes in surficial sediments of coastal ecosystems

J Gong, F Shi, B Ma, J Dong… - Environmental …, 2015 - Wiley Online Library
Little is known about the relative influence of historic processes and environmental gradients
on shaping the diversity of single‐celled eukaryotes in marine benthos. By combining …

To what extent is the DNA of microbial eukaryotes modified during burying into lake sediments? A repeat-coring approach on annually laminated sediments

E Capo, I Domaizon, D Maier, D Debroas… - Journal of …, 2017 - Springer
Paleogenetics provides a powerful framework to reconstruct the long-term temporal
dynamics of various biological groups from aquatic sediments. However, validations are still …