CRISPR–Cas9 genome editing in human cells occurs via the Fanconi anemia pathway
CD Richardson, KR Kazane, SJ Feng, E Zelin… - Nature …, 2018 - nature.com
CRISPR–Cas genome editing creates targeted DNA double-strand breaks (DSBs) that are
processed by cellular repair pathways, including the incorporation of exogenous DNA via …
processed by cellular repair pathways, including the incorporation of exogenous DNA via …
Global detection of DNA repair outcomes induced by CRISPR–Cas9
CRISPR–Cas9 generates double-stranded DNA breaks (DSBs) to activate cellular DNA
repair pathways for genome editing. The repair of DSBs leads to small insertions or …
repair pathways for genome editing. The repair of DSBs leads to small insertions or …
Fanconi anemia gene editing by the CRISPR/Cas9 system
Genome engineering with designer nucleases is a rapidly progressing field, and the ability
to correct human gene mutations in situ is highly desirable. We employed fibroblasts derived …
to correct human gene mutations in situ is highly desirable. We employed fibroblasts derived …
DNA repair pathway choices in CRISPR-Cas9-mediated genome editing
Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements
CRISPR–Cas9 is poised to become the gene editing tool of choice in clinical contexts. Thus
far, exploration of Cas9-induced genetic alterations has been limited to the immediate …
far, exploration of Cas9-induced genetic alterations has been limited to the immediate …
Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair
N Savic, FCAS Ringnalda, H Lindsay, C Berk… - elife, 2018 - elifesciences.org
The CRISPR-Cas9 targeted nuclease technology allows the insertion of genetic
modifications with single base-pair precision. The preference of mammalian cells to repair …
modifications with single base-pair precision. The preference of mammalian cells to repair …
Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus
A Bothmer, T Phadke, LA Barrera, CM Margulies… - Nature …, 2017 - nature.com
The CRISPR–Cas9 system provides a versatile toolkit for genome engineering that can
introduce various DNA lesions at specific genomic locations. However, a better …
introduce various DNA lesions at specific genomic locations. However, a better …
Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
BM Sansbury, AM Hewes, EB Kmiec - Communications biology, 2019 - nature.com
As CRISPR-Cas systems advance toward clinical application, it is essential to identify all the
outcomes of gene-editing activity in human cells. Reports highlighting the remarkable …
outcomes of gene-editing activity in human cells. Reports highlighting the remarkable …
CRISPR/Cas9 genome editing in human hematopoietic stem cells
Genome editing via homologous recombination (HR)(gene targeting) in human
hematopoietic stem cells (HSCs) has the power to reveal gene–function relationships and …
hematopoietic stem cells (HSCs) has the power to reveal gene–function relationships and …
Predicting the mutations generated by repair of Cas9-induced double-strand breaks
The DNA mutation produced by cellular repair of a CRISPR–Cas9-generated double-strand
break determines its phenotypic effect. It is known that the mutational outcomes are not …
break determines its phenotypic effect. It is known that the mutational outcomes are not …