… gene editing may involve nonhomologous end-joining to create various insertion/deletions
(indels) or may employ homologous recombination to modify precisely the target DNA … gene …

… repair (HDR), and nonhomologous end-joining (NHEJ). We owe much of our understanding
of these repair mechanisms to the study of induced DNA cleavage … specific cleavage systems…

… DSB repair is known to be error-prone; however, there are few studies that delve into it deeply
in endogenous genes. Here, we quantify the degree of NHEJ-based DSB repair accuracy (…

… To obtain information on the location of the repair gene, 10 pag of cosmid 43-34 DNA were
cleaved with various restriction endonucleases (see text) and co-transfected with pSV,gptH to …

… The rates of inactivation of a single genetic marker and of a four-marker linkage group … and
the repaired DNA of both samples. Neutral sucrose gradients of mildly digested DNA showed …

… We introduced an N-end rule resistant mutation (D232S) into the chromosomal rad21
gene and examined the level of Rad21 cleavage product. In this background, the level of the …

… The DNA cleavage/ligation reaction of topoisomerase II is the … This article will familiarize
the reader with the DNA cleavage/… –DNA linkage maintains the integrity of the genetic material …

… was mixed with Cch1 or DTT and the combination for 30 min at 0 C; (B) DNA cleavage in …
to calculate the relative level of plasmid DNA cleavage in each sample. Error bars represent …

… I cleavage occurred in at least 70% of tile second genes, and the repair products of these
cleaved genes frequently had small deletions around the cleavage site. Titus, chromosomal …

… Evolution has developed a highly conserved pathway to repair DNA … The DNA backbone
is cleaved by either a DNA AP … The OGG1 gene was shown to encode an enzyme that could …