Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition
BP Kleinstiver, MS Prew, SQ Tsai, NT Nguyen… - Nature …, 2015 - nature.com
CRISPR-Cas9 nucleases target specific DNA sequences using a guide RNA but also
require recognition of a protospacer adjacent motif (PAM) by the Cas9 protein. Although …
require recognition of a protospacer adjacent motif (PAM) by the Cas9 protein. Although …
Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity
Y Tan, AHY Chu, S Bao, DA Hoang… - Proceedings of the …, 2019 - National Acad Sciences
RNA-guided CRISPR-Cas9 proteins have been widely used for genome editing, but their off-
target activities limit broad application. The minimal Cas9 ortholog from Staphylococcus …
target activities limit broad application. The minimal Cas9 ortholog from Staphylococcus …
A Cas9 with PAM recognition for adenine dinucleotides
P Chatterjee, J Lee, L Nip, SRT Koseki… - Nature …, 2020 - nature.com
Abstract CRISPR-associated (Cas) DNA-endonucleases are remarkably effective tools for
genome engineering, but have limited target ranges due to their protospacer adjacent motif …
genome engineering, but have limited target ranges due to their protospacer adjacent motif …
A highly specific SpCas9 variant is identified by in vivo screening in yeast
A Casini, M Olivieri, G Petris, C Montagna… - Nature …, 2018 - nature.com
Despite the utility of CRISPR–Cas9 nucleases for genome editing, the potential for off-target
activity limits their application, especially for therapeutic purposes,. We developed a yeast …
activity limits their application, especially for therapeutic purposes,. We developed a yeast …
Minimal PAM specificity of a highly similar SpCas9 ortholog
P Chatterjee, N Jakimo, JM Jacobson - Science advances, 2018 - science.org
RNA-guided DNA endonucleases of the CRISPR-Cas system are widely used for genome
engineering and thus have numerous applications in a wide variety of fields. CRISPR …
engineering and thus have numerous applications in a wide variety of fields. CRISPR …
Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
Manipulation of DNA by CRISPR-Cas enzymes requires the recognition of a protospacer-
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
Abstract Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-guided
genome editing and transcription regulation in eukaryotic cells, yet their in vivo target …
genome editing and transcription regulation in eukaryotic cells, yet their in vivo target …
High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
CRISPR–Cas9 nucleases are widely used for genome editing but can induce unwanted off-
target mutations. Existing strategies for reducing genome-wide off-target effects of the widely …
target mutations. Existing strategies for reducing genome-wide off-target effects of the widely …
Mapping the genomic landscape of CRISPR–Cas9 cleavage
P Cameron, CK Fuller, PD Donohoue, BN Jones… - Nature …, 2017 - nature.com
RNA-guided CRISPR–Cas9 endonucleases are widely used for genome engineering, but
our understanding of Cas9 specificity remains incomplete. Here, we developed a …
our understanding of Cas9 specificity remains incomplete. Here, we developed a …
Engineered CRISPR-Cas9 nuclease with expanded targeting space
The RNA-guided endonuclease Cas9 cleaves its target DNA and is a powerful genome-
editing tool. However, the widely used Streptococcus pyogenes Cas9 enzyme (SpCas9) …
editing tool. However, the widely used Streptococcus pyogenes Cas9 enzyme (SpCas9) …