Accuracy and clinical implications of seven 25-hydroxyvitamin D methods compared with liquid chromatography–tandem mass spectrometry as a reference

HJ Roth, H Schmidt-Gayk, H Weber… - Annals of clinical …, 2008 - journals.sagepub.com
Background The most reliable assessment of vitamin D status is measurement of plasma 25-
hydroxyvitamin D (25 [OH] D) concentration. High variability in 25 (OH) D measurements …

Current status of clinical 25-hydroxyvitamin D measurement: an assessment of between-laboratory agreement

N Binkley, DC Krueger, S Morgan, D Wiebe - Clinica Chimica Acta, 2010 - Elsevier
BACKGROUND: Historically, methodological differences and lack of standardization led to
between-laboratory variability in 25 (OH) D results. Recent observations raised concern …

[HTML][HTML] Performance evaluation of four 25-hydroxyvitamin D assays to measure 25-hydroxyvitamin D2

J Freeman, K Wilson, R Spears, V Shalhoub… - Clinical biochemistry, 2015 - Elsevier
Objectives The ability of current immunoassays to accurately measure equimolar amounts of
25 (OH) D 2 and 25 (OH) D 3 has been recently questioned. This study determined serum …

HPLC method for 25-hydroxyvitamin D measurement: comparison with contemporary assays

GL Lensmeyer, DA Wiebe, N Binkley… - Clinical …, 2006 - academic.oup.com
Background: The concentration of 25-hydroxyvitamin D [25 (OH) D] in serum has been
designated the functional indicator of vitamin D (VitD) nutritional status. Unfortunately …

25-hydroxy-Vitamin D status: limitations in comparison and clinical interpretation of serum-levels across different assay methods.

D Enko, L Fridrich, E Rezanka, R Stolba, J Ernst… - Clinical …, 2014 - europepmc.org
Background: Over the last decade, clinical interest to evaluate human 25-hydroxy-vitamin D
(25 [OH] D) serum levels has increased exponentially. In the present study, four …

Comparison of four current 25-hydroxyvitamin D assays

HW Moon, JH Cho, M Hur, J Song, GY Oh, CM Park… - Clinical …, 2012 - Elsevier
OBJECTIVES: The performance of recently developed vitamin D total assays (ADVIA
Centaur and Elecsys) was compared to that of liquid chromatography–tandem mass …

Correlation among 25-hydroxy-vitamin D assays

N Binkley, D Krueger, D Gemar… - The Journal of Clinical …, 2008 - academic.oup.com
Context: Measurement of circulating 25-hydroxy-vitamin D [25 (OH) D]) is the accepted
clinical indicator of vitamin D status. However, between-laboratory differences in …

Quantification of 1α, 25-dihydroxy vitamin D by immunoextraction and liquid chromatography–tandem mass spectrometry

FG Strathmann, TJ Laha, AN Hoofnagle - Clinical chemistry, 2011 - academic.oup.com
BACKGROUND 1α, 25-dihydroxy vitamin D [1, 25 (OH) 2D] is the active metabolite of
vitamin D. Antibody-based detection methods lack specificity, but when combined with …

Current assays overestimate 25-hydroxyvitamin D3 and underestimate 25-hydroxyvitamin D2 compared with HPLC: need for assay-specific decision limits and …

P Glendenning, M Taranto, JM Noble… - Annals of clinical …, 2006 - journals.sagepub.com
Background: Clinical demand for quick, cheap, precise and accurate 25-hydroxyvitamin D
(25 (OH) D) results has led to the development of a variety of assay methods. Lack of …

An evaluation of automated methods for measurement of serum 25-hydroxyvitamin D

D Wagner, HEC Hanwell, R Vieth - Clinical biochemistry, 2009 - Elsevier
OBJECTIVES: To compare two new automated assays with the well-established reference
method, DiaSorin radioimmunoassay (RIA), for quantitation of serum total 25-hydroxyvitamin …