CRISPR–Cas9 nucleases are widely used for genome editing but can induce unwanted off-
target mutations. Existing strategies for reducing genome-wide off-target effects of the widely …

Despite the utility of CRISPR–Cas9 nucleases for genome editing, the potential for off-target
activity limits their application, especially for therapeutic purposes,. We developed a yeast …

Abstract The RNA-guided CRISPR–Cas9 nuclease from Streptococcus pyogenes (SpCas9)
has been widely repurposed for genome editing,,,. High-fidelity (SpCas9-HF1) and …

Although RNA-guided genome editing via the CRISPR-Cas9 system is now widely used in
biomedical research, genome-wide target specificities of Cas9 nucleases remain …

Although CRISPR-Cas9 nucleases are widely used for genome editing,, the range of
sequences that Cas9 can recognize is constrained by the need for a specific protospacer …

Manipulation of DNA by CRISPR-Cas enzymes requires the recognition of a protospacer-
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …

CRISPR-Cas9 nucleases target specific DNA sequences using a guide RNA but also
require recognition of a protospacer adjacent motif (PAM) by the Cas9 protein. Although …

Engineered SpCas9s and AsCas12a cleave fewer off-target genomic sites than natural
SpCas9. However, understanding their fidelity, mechanisms and cleavage outcomes …

RNA-guided CRISPR–Cas9 endonucleases are widely used for genome engineering, but
our understanding of Cas9 specificity remains incomplete. Here, we developed a …

RNA-guided CRISPR-Cas9 proteins have been widely used for genome editing, but their off-
target activities limit broad application. The minimal Cas9 ortholog from Staphylococcus …