Manipulation of DNA by CRISPR-Cas enzymes requires the recognition of a protospacer-
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …

The RNA-guided endonuclease Cas9 cleaves its target DNA and is a powerful genome-
editing tool. However, the widely used Streptococcus pyogenes Cas9 enzyme (SpCas9) …

CRISPR-Cas9 nucleases target specific DNA sequences using a guide RNA but also
require recognition of a protospacer adjacent motif (PAM) by the Cas9 protein. Although …

CRISPR–Cas9 nucleases are widely used for genome editing but can induce unwanted off-
target mutations. Existing strategies for reducing genome-wide off-target effects of the widely …

CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-
programmed target site, limiting their sequence accessibility for robust genome editing …

Abstract CRISPR-associated (Cas) DNA-endonucleases are remarkably effective tools for
genome engineering, but have limited target ranges due to their protospacer adjacent motif …

Although CRISPR-Cas9 nucleases are widely used for genome editing,, the range of
sequences that Cas9 can recognize is constrained by the need for a specific protospacer …

Despite the utility of CRISPR–Cas9 nucleases for genome editing, the potential for off-target
activity limits their application, especially for therapeutic purposes,. We developed a yeast …

RNA-guided DNA endonucleases of the CRISPR-Cas system are widely used for genome
engineering and thus have numerous applications in a wide variety of fields. CRISPR …

Abstract Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-guided
genome editing and transcription regulation in eukaryotic cells, yet their in vivo target …