Context: We have recently introduced liquid chromatography-tandem mass spectrometry
(LC-MS/MS) for 25-hydroxyvitamin D2 (25OHD2) and 25OHD3 testing. During subsequent …

Context: Epimers have identical molecular structure but differ in stereochemical
configuration. It is widely believed that the C-3 epimer of 25-hydroxyvitamin D3 [3-epi-25 …

BACKGROUND: The implementation of mass spectrometry to measure serum 25-
hydroxyvitamin D [25 (OH) D] concentrations has led to concerns regarding the …

Background: Since the introduction of liquid chromatography-mass spectrometry (LC/MS) for
assessing vitamin D status, it has been shown that the C-3 epimer can account for a …

With an ever-increasing clinical interest in vitamin D insufficiency, numerous automated
immunoassays, protein binding assays, and in-house LC-MS/MS methods are being …

The prevalence of vitamin D deficiency in the general population has become a major public
health problem. Vitamin D deficiency might have significant consequences not only to bone …

Context: Measurement of circulating 25-hydroxy-vitamin D [25 (OH) D]) is the accepted
clinical indicator of vitamin D status. However, between-laboratory differences in …

Background: Measurement of 25-hydroxyvitamin D2 and D3 (25-OH D2 and D3) is essential
for investigating vitamin D deficiency. Competitive binding techniques are unable to …

Abstract Background An LC–MS/MS method was developed for simultaneous quantification
of 25-hydroxyvitamin D 3 (25 (OH) D 3), 3-epi-25 (OH) D 3, and 25 (OH) D 2 in human …

Hypovitaminosis D is a highly prevalent condition and quantification of serum 25-
hydroxyvitamin D3 is accepted to be the most useful marker for the assessment of the …