… cellular response to topographical contact guidance. We find that for fibroblasts, while focal
adhesions and actin … To disrupt microtubules and inhibit microtubule dynamics we added 1 ​…

… mature linear cell–cell contact (C), at the nascent cell–cell contact (D, … agents that act at
defined points along endocytosis and endosome trafficking pathways. We first disrupted the actin …

… loads acting on cells, to provide an interpretation of how the effects … daughter cell keeps its
contact with the supporting cell while … differential fates in human embryonic stem cell-derived …

… in accordance with the guidelines of the Institutional Animal and … a previously described
soft-contact lithography technique. Briefly, … We first used actin staining (white arrows) to illustrate …

… , embryonic stem cells could be manipulated to form any cell … and contact guidance to this
topography resulted in reduced cell … by disruption of the actin cytoskeleton (Figure 3A). (73) …

… hES cell aggregates with EGTA to disrupt cell–cell interaction… The present study adheres
to the guidelines for hES cell … cells or the current state of the plasma membrane or actin …

… Silgard-184 silicone elastomer base and the curing agents were purchased from Dow …
end-points cells were fixed and stained to allow visualization of the actin cytoskeleton and the …

… the effect of cytochalasin D, which inhibits actin polymerization by … , actin and myosin are all
important for stem cell functioning and … Thus, disrupting the contact guidance of the fiber-like …

… famous phenomenon might be the contact guidance of cells: namely, cell alignment on an
… addition of actin-disrupting agents attenuated the alignment of human embryonic stem cells (…