A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay

CM Hughes, SEM Lewis… - MHR: Basic science …, 1996 - academic.oup.com
… This present study employs a slightly modified alkaline comet assay to compare baseline
DNA damage in spermatozoa from fertile and infertile men. The effects of DNA damaging …

Reliability of the comet assay in cryopreserved human sperm

SM Duty, NP Singh, L Ryan, Z Chen… - Human …, 2002 - academic.oup.com
comet assay using cryopreserved sperm were reproducible and comparable with those
obtained from fresh human sperm. … of the unique packaging of sperm DNA which protects it from …

Oestrogenic compounds and oxidative stress (in human sperm and lymphocytes in the Comet assay)

D Anderson, TE Schmid, A Baumgartner… - … Research/Reviews in …, 2003 - Elsevier
… We examined human sperm and lymphocytes after treatment with six oestrogenic compounds
in the Comet assay, which measures DNA damage, and observed that all caused damage …

DNA Damage in Human and Mouse Spermatozoa after In Vitro-Irradiation Assessed by the Comet Assay

G Haines, B Marples, P Daniel, I Morris - Reproductive Toxicology: In Vitro …, 1998 - Springer
… at post-mortem or human spermatozoa provided by donors … for both alkaline and neutral
comet assays. Under neutral … dose for both murine and human spermatozoa. Similarly, using …

Application of the comet assay for the evaluation of DNA damage in mature sperm

G Gajski, S Ravlić, R Godschalk, A Collins… - … Research/Reviews in …, 2021 - Elsevier
… in sperm is understudied and deserves further attention. In summary, the comet assay can
… tool to study effects of genotoxic exposures on sperm DNA integrity in animals and humans. …

Human semen quality and sperm DNA damage assessed bycomet assay in clinical groups

MH Ramzan, M Ramzan, MM Khan… - Turkish journal of …, 2015 - journals.tubitak.gov.tr
sperm DNA fragmentation between infertile and fertile subjects by using a comet assay. …
Effects of semen analysis on human sperm movement parameters at different times after semen …

Sperm DNA damage measured by comet assay

L Simon, DT Carrell - Spermatogenesis: Methods and Protocols, 2013 - Springer
… The alkaline Comet assay can be used in all cell types including sperm (9). The assay
requires only a few cells; and the level … Human sperm and male infertility. Mutat Res 375:93–104 …

A two-tailed Comet assay for assessing DNA damage in spermatozoa

M Enciso, J Sarasa, A Agarwal, JL Fernández… - Reproductive …, 2009 - Elsevier
… A modification of the original Comet assay was developed for the simultaneous evaluation
of DNA SSB and DSB in human spermatozoa. The 2T-Comet assay is a fast, sensitive, and …

The relationship between environmental exposures to phthalates and DNA damage in human sperm using the neutral comet assay.

SM Duty, NP Singh, MJ Silva, DB Barr… - Environmental …, 2003 - ehp.niehs.nih.gov
… Few published human studies have examined … sperm as measured by the comet assay. In
the present study, we used the neutral comet assay to measure DNA integrity in human sperm

The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development

ID Morris, S Ilott, L Dixon, DR Brison - Human reproduction, 2002 - academic.oup.com
… have hindered the development of the Comet assays for comparative studies of male …
Comet assay to provide quantitative measures of DNA damage in human and murine sperm (…