[HTML][HTML] Analysis of RNA modifications by second-and third-generation deep sequencing: 2020 update
Y Motorin, V Marchand - Genes, 2021 - mdpi.com
… , direct RNA modification analysis by nanopore sequencing is … in RNA epitranscriptomics.
Thus, classical approaches are still widely used in the routine analysis of RNA modifications, …
Thus, classical approaches are still widely used in the routine analysis of RNA modifications, …
[HTML][HTML] Methods for RNA modification mapping using deep sequencing: established and new emerging technologies
… RNA species. In this brief overview, we consider techniques that utilize deep sequencing
for modification calling, … of RNA modifications ranges from antibody-(Ab)-based anti-methylated/…
for modification calling, … of RNA modifications ranges from antibody-(Ab)-based anti-methylated/…
Traces of post-transcriptional RNA modifications in deep sequencing data
… modification, maturation and degradation processes. Here, we focus on small RNA sequencing
… well known modified sites in tRNAs, we provide evidence that modified nucleotides are a …
… well known modified sites in tRNAs, we provide evidence that modified nucleotides are a …
Epitranscriptome sequencing technologies: decoding RNA modifications
… of RNA modifications. More recently, a method that detects inosine sites based only on RNA-seq
… of publicly available RNA-seq data and thus does not require deep sequencing of both …
… of publicly available RNA-seq data and thus does not require deep sequencing of both …
Going global: the new era of mapping modifications in RNA
PA Limbach, MJ Paulines - … Interdisciplinary Reviews: RNA, 2017 - Wiley Online Library
… sequencing technologies, the use of high-throughput RNA-Seq10 has enabled the study of
some RNA modifications at … and deep sequencing that uses a chemical biology approach to …
some RNA modifications at … and deep sequencing that uses a chemical biology approach to …
Fingerprints of modified RNA bases from deep sequencing profiles
… deep sequencing can distinguish several RNA base modifications in a single sequencing
… Our current methods employed ten different common RNA modifications, including all those …
… Our current methods employed ten different common RNA modifications, including all those …
AlkAniline‐Seq: Profiling of m7G and m3C RNA Modifications at Single Nucleotide Resolution
V Marchand, L Ayadi, FGM Ernst… - Angewandte Chemie …, 2018 - Wiley Online Library
… Deep sequencing was applied in a new concept of library preparation for detection of
RNA modifications. Modified RNA is treated by alkaline hydrolysis, dephosphorylated, and …
RNA modifications. Modified RNA is treated by alkaline hydrolysis, dephosphorylated, and …
Repurposing RNA sequencing for discovery of RNA modifications in clinical cohorts
… types of base pair–disrupting RNA modifications and also captured by standard RNA-seq,
we used residual ribosomal RNA (rRNA) reads, present in most RNA-seq libraries (fig. S3), as …
we used residual ribosomal RNA (rRNA) reads, present in most RNA-seq libraries (fig. S3), as …
General principles and limitations for detection of RNA modifications by sequencing
… a sequencing-by-synthesis type of analysis. Thus, a particular behavior of RNA modified …
for their detection (and quantification) by deep sequencing, and RT properties have great …
for their detection (and quantification) by deep sequencing, and RT properties have great …
[HTML][HTML] Pseudouridine in a new era of RNA modifications
… , researchers developed Pseudo-seq by labeling Ψ in uniformly fragmented RNA by CMC, …
the modified Ψ sites. Subsequent circularization, amplification and deep sequencing revealed …
the modified Ψ sites. Subsequent circularization, amplification and deep sequencing revealed …
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