[HTML][HTML] Analysis of RNA modifications by second-and third-generation deep sequencing: 2020 update

Y Motorin, V Marchand - Genes, 2021 - mdpi.com
… , direct RNA modification analysis by nanopore sequencing is … in RNA epitranscriptomics.
Thus, classical approaches are still widely used in the routine analysis of RNA modifications, …

[HTML][HTML] Methods for RNA modification mapping using deep sequencing: established and new emerging technologies

Y Motorin, M Helm - Genes, 2019 - mdpi.com
RNA species. In this brief overview, we consider techniques that utilize deep sequencing
for modification calling, … of RNA modifications ranges from antibody-(Ab)-based anti-methylated/…

Traces of post-transcriptional RNA modifications in deep sequencing data

S Findeiß, D Langenberger, PF Stadler, S Hoffmann - 2011 - degruyter.com
modification, maturation and degradation processes. Here, we focus on small RNA sequencing
… well known modified sites in tRNAs, we provide evidence that modified nucleotides are a …

Epitranscriptome sequencing technologies: decoding RNA modifications

X Li, X Xiong, C Yi - Nature methods, 2017 - nature.com
… of RNA modifications. More recently, a method that detects inosine sites based only on RNA-seq
… of publicly available RNA-seq data and thus does not require deep sequencing of both …

Going global: the new era of mapping modifications in RNA

PA Limbach, MJ Paulines - … Interdisciplinary Reviews: RNA, 2017 - Wiley Online Library
sequencing technologies, the use of high-throughput RNA-Seq10 has enabled the study of
some RNA modifications at … and deep sequencing that uses a chemical biology approach to …

Fingerprints of modified RNA bases from deep sequencing profiles

AM Kietrys, WA Velema, ET Kool - Journal of the American …, 2017 - ACS Publications
deep sequencing can distinguish several RNA base modifications in a single sequencing
… Our current methods employed ten different common RNA modifications, including all those …

AlkAniline‐Seq: Profiling of m7G and m3C RNA Modifications at Single Nucleotide Resolution

V Marchand, L Ayadi, FGM Ernst… - Angewandte Chemie …, 2018 - Wiley Online Library
Deep sequencing was applied in a new concept of library preparation for detection of
RNA modifications. Modified RNA is treated by alkaline hydrolysis, dephosphorylated, and …

Repurposing RNA sequencing for discovery of RNA modifications in clinical cohorts

KT Tan, LW Ding, CS Wu, DG Tenen, H Yang - Science Advances, 2021 - science.org
… types of base pair–disrupting RNA modifications and also captured by standard RNA-seq,
we used residual ribosomal RNA (rRNA) reads, present in most RNA-seq libraries (fig. S3), as …

General principles and limitations for detection of RNA modifications by sequencing

Y Motorin, M Helm - Accounts of Chemical Research, 2023 - ACS Publications
… a sequencing-by-synthesis type of analysis. Thus, a particular behavior of RNA modified
for their detection (and quantification) by deep sequencing, and RT properties have great …

[HTML][HTML] Pseudouridine in a new era of RNA modifications

BS Zhao, C He - Cell research, 2015 - nature.com
… , researchers developed Pseudo-seq by labeling Ψ in uniformly fragmented RNA by CMC, …
the modified Ψ sites. Subsequent circularization, amplification and deep sequencing revealed …