… mouse utricle via canalostomy. We found that AAV8 was the most effective AAV vector in
utricular gene transfer… In the normal utricle after AAV8 infusion, transduction efficiency peaked at …

… genes E1, E3, the viral polymerase, and the preterminal protein. We are currently working
to characterize viral-mediated gene transfer into hair cells of the cultured mouse utricle using …

… cell quiescence in whole organ cultures of adult mouse utricles. Co-infection of … mouse
utricles with separate adenoviruses encoding mouse Oct3/4 (Ad.O, 5×10 7 TU/mL), mouse …

… -mediated gene transfer is an efficient method of gene over-expression in the vestibular end
organs. However, AAV has limited usefulness for delivering a large gene, or multiple genes, …

… To evaluate exogenous gene expression as a function of time post-injection, we injected 15
wild-type P1 mice via the utricle with 1 … Gene transfer with AAV9-PHP.B rescues hearing in a …

… ear utricle requires mechanosensory hair cells (HCs) to detect linear acceleration. After damage,
non-mammalian utricles … in neonatal mouse utricle activates the Wnt target gene Lgr5 in …

… these characteristics using genetic labeling mediated by Fgf8 mcm gene-targeted mice and
… Fgf8 mcm expression initiates at E11.5 in the future striolar region of the utricle, labeling hair …

… of utricles from adult mice (Figure 1) 1-6 . The utricle is a vestibular organ, and the hair cells
of the utricle … The adult mouse utricle preparation represents a mature sensory epithelium for …

… In this study, we noticed that the utricle epithelia of triallelic neonatal mouse induced from
P0 to P4 (P0–P4:P6) became much thicker than the controls (Figs. 1, 2 and 3). This …

… This study introduces a new method for efficient conditional hair cell ablation in adult mouse
utricles and demonstrates that hair cells are spontaneously regenerated in vivo in regions …