Studies on the metal binding sites in the catalytic domain of β1, 4-galactosyltransferase
E Boeggeman, PK Qasba - Glycobiology, 2002 - academic.oup.com
The catalytic domain of bovine β1, 4-galactosyltransferase (β4Gal-T1) has been shown to
have two metal binding sites, each with a distinct binding affinity. Site I binds Mn2+ with high …
have two metal binding sites, each with a distinct binding affinity. Site I binds Mn2+ with high …
[HTML][HTML] Crystal structures of the bovine β4galactosyltransferase catalytic domain and its complex with uridine diphosphogalactose
LN Gastinel, C Cambillau, Y Bourne - The EMBO Journal, 1999 - embopress.org
Abstract β1, 4‐galactosyltransferase T1 (β4Gal‐T1, EC 2.4. 1.90/38), a Golgi resident
membrane‐bound enzyme, transfers galactose from uridine diphosphogalactose to the …
membrane‐bound enzyme, transfers galactose from uridine diphosphogalactose to the …
Crystal structure of the catalytic domain of Drosophila β1, 4-galactosyltransferase-7
B Ramakrishnan, PK Qasba - Journal of Biological Chemistry, 2010 - ASBMB
The β1, 4-galactosyltransferase-7 (β4Gal-T7) enzyme, one of seven members of the β4Gal-T
family, transfers in the presence of manganese Gal from UDP-Gal to an acceptor sugar …
family, transfers in the presence of manganese Gal from UDP-Gal to an acceptor sugar …
Effect of the Met344His mutation on the conformational dynamics of bovine β-1, 4-galactosyltransferase: crystal structure of the Met344His mutant in complex with …
B Ramakrishnan, E Boeggeman, PK Qasba - Biochemistry, 2004 - ACS Publications
β-1, 4-Galactosyltransferase (β4Gal-T1) in the presence of manganese ion transfers
galactose from UDP-galactose (UDP-Gal) to N-acetylglucosamine (GlcNAc) that is either …
galactose from UDP-galactose (UDP-Gal) to N-acetylglucosamine (GlcNAc) that is either …
Mutation of Arginine 228 to Lysine Enhances the Glucosyltransferase Activity of Bovine β-1,4-Galactosyltransferase I,,
B Ramakrishnan, E Boeggeman, PK Qasba - Biochemistry, 2005 - ACS Publications
β-1, 4-Galactosyltransferase I (β4Gal-T1) normally transfers Gal from UDP-Gal to GlcNAc in
the presence of Mn2+ ion (Gal-T activity) and also transfers Glc from UDP-Glc to GlcNAc (Glc …
the presence of Mn2+ ion (Gal-T activity) and also transfers Glc from UDP-Glc to GlcNAc (Glc …
Structural snapshots of β-1, 4-galactosyltransferase-I along the kinetic pathway
B Ramakrishnan, V Ramasamy, PK Qasba - Journal of molecular biology, 2006 - Elsevier
During the catalytic cycle of β1, 4-galactosyltransferase-1 (Gal-T1), upon the binding of
Mn2+ followed by UDP–Gal, two flexible loops, a long and a short loop, change their …
Mn2+ followed by UDP–Gal, two flexible loops, a long and a short loop, change their …
Structure and catalytic cycle of β-1, 4-galactosyltransferase
B Ramakrishnan, E Boeggeman, V Ramasamy… - Current opinion in …, 2004 - Elsevier
β-1, 4-galactosyltransferase-1, a housekeeping enzyme that functions in the synthesis of
glycoconjugates, has two flexible loops, one short and one long. Upon binding a metal ion …
glycoconjugates, has two flexible loops, one short and one long. Upon binding a metal ion …
Structure-based design of β1, 4-galactosyltransferase I (β4Gal-T1) with equally efficient N-acetylgalactosaminyltransferase activity: point mutation broadens β4Gal-T1 …
B Ramakrishnan, PK Qasba - Journal of Biological Chemistry, 2002 - ASBMB
β1, 4-Galactosyltransferase I (Gal-T1) normally transfers Gal from UDP-Gal to GlcNAc in the
presence of Mn 2+ ion. In the presence of α-lactalbumin (LA), the Gal acceptor specificity is …
presence of Mn 2+ ion. In the presence of α-lactalbumin (LA), the Gal acceptor specificity is …
Comparison of the Closed Conformation of the β1, 4-galactosyltransferase-1 (β4Gal-T1) in the Presence and Absence of α-lactalbumin (LA)
B Ramakrishnan, PK Qasba - Journal of Biomolecular Structure …, 2003 - Taylor & Francis
Abstract β1, 4-Galactosyltransferase (β4Gal-T1) transfers galactose from UDP-galactose to
N-acetyl-glucosamine (GlcNAc) in the presence of Mn2+ ion. However, in the presence of α …
N-acetyl-glucosamine (GlcNAc) in the presence of Mn2+ ion. However, in the presence of α …
Role of a conserved acidic cluster in bovine β1, 4 galactosyltransferase-1 probed by mutagenesis of a bacterially expressed recombinant enzyme
Y Zhang, VA Malinovskii, TJ Fiedler, K Brew - Glycobiology, 1999 - academic.oup.com
The truncated catalytic domain of bovine β1, 4 galactosyltransferase-1 was expressed as
inclusion bodies in E. coli and folded to generate 10–15 mg of active enzyme per liter of …
inclusion bodies in E. coli and folded to generate 10–15 mg of active enzyme per liter of …