[HTML][HTML] A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation

AC Neumann-Cip, V Fingerle, G Margos… - Frontiers in …, 2020 - frontiersin.org
The genus Borrelia comprises vector-borne bacterial pathogens that can severely affect
human and animal health. Members of the Borrelia burgdorferi sensu lato species complex …

[HTML][HTML] Identification of Borrelia Species after Creation of an In-House MALDI-TOF MS Database

A Calderaro, C Gorrini, G Piccolo, S Montecchini… - PLoS …, 2014 - journals.plos.org
Lyme borreliosis (LB) is a multisystemic disease caused by Borrelia burgdorferi sensu lato
(sl) complex transmitted to humans by Ixodes ticks. B. burgdorferi sl complex, currently …

Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato Using Molecular Biological Methods

G Margos, I Notter, V Fingerle - Borrelia burgdorferi: Methods and …, 2018 - Springer
Bacterial species identification is required in different disciplines and—depending on the
purpose—levels of specificity or resolution of typing may vary. Nowadays, molecular …

Evaluation of real-time PCR targeting hbb gene for Borrelia species identification

J Ferdin, T Cerar, F Strle, E Ružić-Sabljić - Journal of microbiological …, 2010 - Elsevier
Several molecular methods have been employed for Borrelia species identification. Newly
developed technology, real-time polymerase chain reaction (RT-PCR), combines …

[HTML][HTML] A Novel High Discriminatory Protocol for the Detection of Borrelia afzelii, Borrelia burgdorferi Sensu Stricto and Borrelia garinii in Ticks

G Chiappa, M Perini, A Cafiso, R Nodari… - Pathogens, 2022 - mdpi.com
Bacteria of the Borrelia burgdorferi sensu lato complex are the causative agents of Lyme
borreliosis (LB). Even if the conventional diagnosis of LB does not rely on the species itself …

A novel duplex real-time PCR permits simultaneous detection and differentiation of Borrelia miyamotoi and Borrelia burgdorferi sensu lato

R Venczel, L Knoke, M Pavlovic, E Dzaferovic… - Infection, 2016 - Springer
Purpose For simultaneous detection of Borrelia miyamotoi (relapsing fever spirochete) and
Borrelia burgdorferi sensu lato, we have developed a duplex real-time PCR targeting the …

[HTML][HTML] MALDI-TOF Mass Spectrometry Detection of Pathogens in Vectors: The Borrelia crocidurae/Ornithodoros sonrai Paradigm

A Fotso Fotso, O Mediannikov, G Diatta… - PLoS neglected …, 2014 - journals.plos.org
Background In Africa, relapsing fever borreliae are neglected vector-borne pathogens that
cause mild to deadly septicemia and miscarriage. Screening vectors for the presence of …

[HTML][HTML] Characterization of Borrelia burgdorferi sensu lato isolates by pulsed-field gel electrophoresis after MluI restriction of genomic DNA

E Ružić-Sabljić, A Zore, F Strle - Research in microbiology, 2008 - Elsevier
At least three Borrelia species (Borrelia afzelii, Borrelia garinii and Borrelia burgdorferi
sensu stricto) cause disease in humans, but Borrelia spielmanii, Borrelia valaisiana, Borrelia …

Development and evaluation of a two-step multiplex TaqMan real-time PCR assay for detection/quantification of different genospecies of Borrelia burgdorferi sensu …

M Nunes, R Parreira, T Carreira, J Inácio… - Ticks and tick-borne …, 2018 - Elsevier
Nowadays, at least four clinically important B. burgdorferi sensu lato (sl) genospecies (B.
afzelii, B. garinii, B. burgdorferi sensu stricto (ss) and B. lusitaniae) circulate in Portugal …

Delineation of Borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of Borrelia spielmanii sp. nov.

D Richter, D Postic, N Sertour, I Livey… - … of systematic and …, 2006 - microbiologyresearch.org
To evaluate multilocus sequence analysis (MLSA) for taxonomic purposes in the delineation
of species within Borrelia burgdorferi sensu lato, seven relevant loci of various strains for …