An active and soluble human β1, 4-galactosyltransferase (β-GT) was produced in
Escherichia coli using a maltose-binding protein fusion system. The purified recombinant β …

β‐1, 4‐Galactosyltransferase (Gal‐T, EC 2.4. 1.38) transfers galactose (Gal) from JDP‐Gal to
N‐acetyl‐d‐glucosamine or a derivative GlcNAc‐R. Soluble Gal‐T, purified from human …

The capacity of UDP-N-acetyl-α-D-glucosamine (UDP-GlcNAc) as an in vitro acceptor
substrate for β-1, 4-galactosyltransferase (β4GalT1, EC 2.4. 1.38) from human and bovine …

A β1, 3-galactosyltransferase (WbgO) was identified in Escherichia coli O55: H7. Its function
was confirmed by radioactive activity assay and structure analysis of the disaccharide …

A protease‐defective strain of Saccharomyces cerevisiae (BT 150) was used to express full‐
length cDNA of HeLa cell β‐D‐N‐acetylglucosaminide‐β‐1, 4‐galactosyltransferase (gal‐T) …

A number of N-and C-terminal deletion and point mutants of bovine β-1, 4
galactosyltransferase (β-1, 4GT) were expressed in E. coli to determine the binding regions …

We report in this communication the first large-scale heterologous expression of a
glycosyltransferase in yeast. A soluble form of a human β-1, 4-galactosyltransferase (EC 2.4 …

A cDNA encoding a β-1, 4-galactosyltransferase named β-1, 4-GalT II was cloned from a
cDNA library of the human breast tumor cell line, MRK-nu-1. Initially, a 860-bp PCR fragment …

We inserted a full‐length murine cDNA, which had been isolated from F9 embryonal
carcinoma cells by using a bovine lactose synthetase A protein cDNA as a probe, in a …

β‐1, 4‐galactosyltransferase 1 (β4gal‐T1, EC 2.4. 1.38) transfers galactose from UDP‐
galactose to free N‐acetyl‐d‐glucosamine or bound N‐acetyl‐d‐glucosamine‐R. Soluble …