Characterization and synthetic application of a novel β1, 3-galactosyltransferase from Escherichia coli O55: H7
A β1, 3-galactosyltransferase (WbgO) was identified in Escherichia coli O55: H7. Its function
was confirmed by radioactive activity assay and structure analysis of the disaccharide …
was confirmed by radioactive activity assay and structure analysis of the disaccharide …
Production and characterization of active soluble human β1, 4-galactosyltransferase in Escherichia coli as a useful catalyst in synthesis of the Gal β1→ 4 GlcNAc …
S Shibatani, K Fujiyama, S Nishiguchi, T Seki… - Journal of bioscience …, 2001 - Elsevier
An active and soluble human β1, 4-galactosyltransferase (β-GT) was produced in
Escherichia coli using a maltose-binding protein fusion system. The purified recombinant β …
Escherichia coli using a maltose-binding protein fusion system. The purified recombinant β …
Highly efficient chemoenzymatic synthesis of β1–4-linked galactosides with promiscuous bacterial β1–4-galactosyltransferases
K Lau, V Thon, H Yu, L Ding, Y Chen… - Chemical …, 2010 - pubs.rsc.org
Two bacterial β1–4-galactosyltransferases, NmLgtB and Hp1–4GalT, exhibit promiscuous
and complementary acceptor substrate specificity. They have been used in an efficient one …
and complementary acceptor substrate specificity. They have been used in an efficient one …
Donor substrate promiscuity of bacterial β1–3-N-acetylglucosaminyltransferases and acceptor substrate flexibility of β1–4-galactosyltransferases
Y Li, M Xue, X Sheng, H Yu, J Zeng, V Thon… - Bioorganic & medicinal …, 2016 - Elsevier
Abstract β1–3-N-Acetylglucosaminyltransferases (β3GlcNAcTs) and β1–4-
galactosyltransferases (β4GalTs) have been broadly used in enzymatic synthesis of N …
galactosyltransferases (β4GalTs) have been broadly used in enzymatic synthesis of N …
Biochemical characterization of the novel α-1, 3-galactosyltransferase WclR from Escherichia coli O3
C Chen, B Liu, Y Xu, N Utkina, D Zhou, L Danilov… - Carbohydrate …, 2016 - Elsevier
Glycosyltransferases (GTs) catalyze the formation of regio-and stereo-specific glycosidic
linkages between specific sugar donors and recipients. In this study, the function of the gene …
linkages between specific sugar donors and recipients. In this study, the function of the gene …
A novel β-galactosidase capable of glycosyl transfer from Enterobacter agglomerans B1
L Lu, M Xiao, X Xu, Z Li, Y Li - Biochemical and biophysical research …, 2007 - Elsevier
A novel transglycosylating β-galactosidase was purified from Enterobacter agglomerans B1.
It was a homodimer of∼ 248kDa. The optimal pH and temperature for oNPGal hydrolysis …
It was a homodimer of∼ 248kDa. The optimal pH and temperature for oNPGal hydrolysis …
Flexibility in the donor substrate specificity of β 1,4-galactosyltransferase: application in the synthesis of complex carbohydrates
MM Palcic, O Hindsgaul - Glycobiology, 1991 - academic.oup.com
Biosynthetically, bovine N-acetylglucosainine β 1, 4-galacto-syltransferase (GalT) catalyses
the transfer of galactosyl residues from UDP-Gal to the 4-position of GlcNAc units, resulting …
the transfer of galactosyl residues from UDP-Gal to the 4-position of GlcNAc units, resulting …
A convenient synthesis of β-D-galactosyl disaccharide derivatives using the β-D-galactosidase from Bacillus circulans
T Usui, S Kubota, H Ohi - Carbohydrate research, 1993 - Elsevier
Abstract β-d-Gal-(1→ 4)-β-d-GlcNAc-OC 6 H 4 NO 2-p (p-nitrophenyl N-acetyl-β-
lactosaminide) and β-d-Gal-(1→ 6)-β-d-GlcNAc-OC 6 H 4 NO 2-p (p-nitrophenyl N-acetyl-β …
lactosaminide) and β-d-Gal-(1→ 6)-β-d-GlcNAc-OC 6 H 4 NO 2-p (p-nitrophenyl N-acetyl-β …
On the preparative use of recombinant β (1–3) galactosyl-transferase
G Baisch, R Öhrlein, M Streiff, F Kolbinger - Bioorganic & medicinal …, 1998 - Elsevier
A number of non-natural N-acyl derivatives of glucosamine is incubated with a recombinant
β (1–3) galactosyl-transferase and UDP-galactose. Surprisingly, the enzyme recognizes the …
β (1–3) galactosyl-transferase and UDP-galactose. Surprisingly, the enzyme recognizes the …
Enzymatic Synthesis of Galactose‐Containing Disaccharides Employing β‐Galactosidase from Bacillus circulans
E Farkas, J Thiem - European journal of organic chemistry, 1999 - Wiley Online Library
Galβ1→ 4 disaccharide structures are vital core units of the oligosaccharide components of
glycoconjugates. β‐Galactosidase from Bacillus circulans (EC 3.2. 1.23) catalyses the …
glycoconjugates. β‐Galactosidase from Bacillus circulans (EC 3.2. 1.23) catalyses the …