Recombinant Soluble β‐1,4‐Galactosyltransferases Expressed in Saccharomyces cerevisiae Purification, Characterization and Comparison with Human Enzyme
M Malissard, L Borsig, S Di Marco… - European journal of …, 1996 - Wiley Online Library
β‐1, 4‐Galactosyltransferase (Gal‐T, EC 2.4. 1.38) transfers galactose (Gal) from JDP‐Gal to
N‐acetyl‐d‐glucosamine or a derivative GlcNAc‐R. Soluble Gal‐T, purified from human …
N‐acetyl‐d‐glucosamine or a derivative GlcNAc‐R. Soluble Gal‐T, purified from human …
Expression of deletion constructs of bovine β-1, 4-galactosyltransferase in Escherichia coli: importance of Cysl34 for its activity
EE Boeggeman, PV Balaji, N Sethi… - Protein Engineering …, 1993 - academic.oup.com
Abstract Bovine β-1, 4-galactosyltransferase (β-1, 4-GT; EC 2.4. 1.90) belongs to the
glycosyltransferase family and as such shares a general topology: an N-terminal …
glycosyltransferase family and as such shares a general topology: an N-terminal …
Localization of the long form of beta-1, 4-galactosyltransferase to the plasma membrane and Golgi complex of 3T3 and F9 cells by immunofluorescence confocal …
A Youakim, DH Dubois… - Proceedings of the …, 1994 - National Acad Sciences
beta-1, 4-Galactosyltransferase (GalTase) is localized to two subcellular compartments, the
Golgi complex, where it participates in cellular glycosylation, and the plasma membrane …
Golgi complex, where it participates in cellular glycosylation, and the plasma membrane …
A phylogenetic view and functional annotation of the animal β1, 3-glycosyltransferases of the GT31 CAZy family
D Petit, RE Teppa, A Harduin-Lepers - Glycobiology, 2021 - academic.oup.com
Abstract The formation of β1, 3-linkages on animal glycoconjugates is catalyzed by a subset
of β1, 3-glycosyltransferases grouped in the Carbohydrate-Active enZYmes family …
of β1, 3-glycosyltransferases grouped in the Carbohydrate-Active enZYmes family …
Characterization of the substrate specificity of α1, 3galactosyltransferase utilizing modified N-acetyllactosamine disaccharides
CLM Stults, BA Macher, R Bhatti, OP Srivastava… - …, 1999 - academic.oup.com
Abstract α1, 3galactosyltransferase (α1, 3GalT) catalyzes the synthesis of a range of
glycoconjugates containing the Galα1, 3Gal epitope which is recognized by the naturally …
glycoconjugates containing the Galα1, 3Gal epitope which is recognized by the naturally …
Rational design, synthesis, and characterization of novel inhibitors for human β1, 4-galactosyltransferase
K Takaya, N Nagahori, M Kurogochi… - Journal of medicinal …, 2005 - ACS Publications
An affinity labeling reagent, uridine 5 '-(6-amino-{2-[(7-bromomethyl-2-naphthyl)
methoxycarbonylmethoxy] ethoxy} acetyl-6-deoxy-α-d-galactopyranosyl) diphosphate (1a) …
methoxycarbonylmethoxy] ethoxy} acetyl-6-deoxy-α-d-galactopyranosyl) diphosphate (1a) …
Molecular cloning of a human cDNA encoding β-1, 4-galactosyltransferase with 37% identity to mammalian UDP-Gal: GlcNAc β-1, 4-galactosyltransferase
T Sato, K Furukawa, H Bakker… - Proceedings of the …, 1998 - National Acad Sciences
A cDNA encoding a β-1, 4-galactosyltransferase named β-1, 4-GalT II was cloned from a
cDNA library of the human breast tumor cell line, MRK-nu-1. Initially, a 860-bp PCR fragment …
cDNA library of the human breast tumor cell line, MRK-nu-1. Initially, a 860-bp PCR fragment …
Structural and functional features of glycosyltransferases
C Breton, J Mucha, C Jeanneau - Biochimie, 2001 - Elsevier
Most of the glycosylation reactions that generate the great diversity of oligosaccharide
structures of eukaryotic cells occur in the Golgi apparatus. This review deals with the most …
structures of eukaryotic cells occur in the Golgi apparatus. This review deals with the most …
Synthesis of a library of variously modified 4-methylumbelliferyl xylosides and a structure–activity study of human β4GalT7
S Dahbi, JC Jacquinet, I Bertin-Jung… - Organic & …, 2017 - pubs.rsc.org
Proteoglycans (PGs) are complex macromolecules that are composed of glycosaminoglycan
(GAG) chains covalently attached to a core protein through a tetrasaccharide linker. The …
(GAG) chains covalently attached to a core protein through a tetrasaccharide linker. The …
Glucosaminylglycan biosynthesis: what we can learn from the X-ray crystal structures of glycosyltransferases GlcAT1 and EXTL2
M Negishi, J Dong, TA Darden, LG Pedersen… - Biochemical and …, 2003 - Elsevier
The X-ray crystal structures of two glycosyltransferases (GTs)—β1, 3-glucuronyltransferase 1
(GlcAT1) and α1, 4-N-acetylhexosaminyltransferase (EXTL2)—have now been determined …
(GlcAT1) and α1, 4-N-acetylhexosaminyltransferase (EXTL2)—have now been determined …