Structural snapshots of β-1, 4-galactosyltransferase-I along the kinetic pathway
B Ramakrishnan, V Ramasamy, PK Qasba - Journal of molecular biology, 2006 - Elsevier
During the catalytic cycle of β1, 4-galactosyltransferase-1 (Gal-T1), upon the binding of
Mn2+ followed by UDP–Gal, two flexible loops, a long and a short loop, change their …
Mn2+ followed by UDP–Gal, two flexible loops, a long and a short loop, change their …
Crystal structures of β-1, 4-galactosyltransferase 7 enzyme reveal conformational changes and substrate binding
Y Tsutsui, B Ramakrishnan, PK Qasba - Journal of Biological Chemistry, 2013 - ASBMB
The β-1, 4-galactosyltransferase 7 (β4GalT7) enzyme is involved in proteoglycan synthesis.
In the presence of a manganese ion, it transfers galactose from UDP-galactose to xylose on …
In the presence of a manganese ion, it transfers galactose from UDP-galactose to xylose on …
Comparison of the Closed Conformation of the β1, 4-galactosyltransferase-1 (β4Gal-T1) in the Presence and Absence of α-lactalbumin (LA)
B Ramakrishnan, PK Qasba - Journal of Biomolecular Structure …, 2003 - Taylor & Francis
Abstract β1, 4-Galactosyltransferase (β4Gal-T1) transfers galactose from UDP-galactose to
N-acetyl-glucosamine (GlcNAc) in the presence of Mn2+ ion. However, in the presence of α …
N-acetyl-glucosamine (GlcNAc) in the presence of Mn2+ ion. However, in the presence of α …
Structure-based design of β1, 4-galactosyltransferase I (β4Gal-T1) with equally efficient N-acetylgalactosaminyltransferase activity: point mutation broadens β4Gal-T1 …
B Ramakrishnan, PK Qasba - Journal of Biological Chemistry, 2002 - ASBMB
β1, 4-Galactosyltransferase I (Gal-T1) normally transfers Gal from UDP-Gal to GlcNAc in the
presence of Mn 2+ ion. In the presence of α-lactalbumin (LA), the Gal acceptor specificity is …
presence of Mn 2+ ion. In the presence of α-lactalbumin (LA), the Gal acceptor specificity is …
Interdependence of backbone flexibility, residue conservation, and enzyme function: A case study on β1, 4-galactosyltransferase-I
K Gunasekaran, B Ma, B Ramakrishnan, PK Qasba… - Biochemistry, 2003 - ACS Publications
β1, 4-Galactosyltransferase-I (β4Gal-T1) catalyzes the transfer of a galactose from UDP-
galactose to N-acetylglucosamine. A recent crystal structure determination of the substrate …
galactose to N-acetylglucosamine. A recent crystal structure determination of the substrate …
Structure of UDP complex of UDP-galactose: β-galactoside-α-1, 3-galactosyltransferase at 1.53-Å resolution reveals a conformational change in the catalytically …
UDP-galactose: β-galactosyl α-1, 3-galactosyltransferase (α3GT) catalyzes the transfer of
galactose from UDP-α-d-galactose into an α-1, 3 linkage with β-galactosyl groups in …
galactose from UDP-α-d-galactose into an α-1, 3 linkage with β-galactosyl groups in …
The role of tryptophan 314 in the conformational changes of β1, 4-galactosyltransferase-I
V Ramasamy, B Ramakrishnan, E Boeggeman… - Journal of molecular …, 2003 - Elsevier
β1, 4-Galactosyltransferase-I (β4Gal-T1) undergoes critical conformational changes upon
substrate binding from an open conformation (conf-I) to the closed conformation (conf-II) …
substrate binding from an open conformation (conf-I) to the closed conformation (conf-II) …
Structure and catalytic cycle of β-1, 4-galactosyltransferase
B Ramakrishnan, E Boeggeman, V Ramasamy… - Current opinion in …, 2004 - Elsevier
β-1, 4-galactosyltransferase-1, a housekeeping enzyme that functions in the synthesis of
glycoconjugates, has two flexible loops, one short and one long. Upon binding a metal ion …
glycoconjugates, has two flexible loops, one short and one long. Upon binding a metal ion …
Crystal structure of β1, 4-galactosyltransferase complex with UDP-Gal reveals an oligosaccharide acceptor binding site
B Ramakrishnan, PV Balaji, PK Qasba - Journal of molecular biology, 2002 - Elsevier
The crystal structure of the catalytic domain of bovine β1, 4-galactosyltransferase (Gal-T1) co-
crystallized with UDP-Gal and MnCl2 has been solved at 2.8 Å resolution. The structure not …
crystallized with UDP-Gal and MnCl2 has been solved at 2.8 Å resolution. The structure not …
[HTML][HTML] Crystal structures of the bovine β4galactosyltransferase catalytic domain and its complex with uridine diphosphogalactose
LN Gastinel, C Cambillau, Y Bourne - The EMBO Journal, 1999 - embopress.org
Abstract β1, 4‐galactosyltransferase T1 (β4Gal‐T1, EC 2.4. 1.90/38), a Golgi resident
membrane‐bound enzyme, transfers galactose from uridine diphosphogalactose to the …
membrane‐bound enzyme, transfers galactose from uridine diphosphogalactose to the …