[HTML][HTML] Establishment of a minor groove binder-probe based quantitative real time PCR to detect Borrelia burgdorferi sensu lato and differentiation of Borrelia …

C Strube, VM Montenegro, C Epe, E Eckelt… - Parasites & vectors, 2010 - Springer
Background Borrelia burgdorferi sensu lato (sl), the causative agent of Lyme borreliosis, is
transmitted by ticks of the genus Ixodes as vector. For identification of Borrelia infections in …

Validation of droplet digital PCR for the detection and absolute quantification of Borrelia DNA in Ixodes scapularis ticks

JL King, AD Smith, EA Mitchell, MS Allen - Parasitology, 2017 - cambridge.org
We evaluated the QX200 Droplet Digital PCR (ddPCR™, Bio-Rad) system and protocols for
the detection of the tick-borne pathogens Borrelia burgdorferi and Borrelia miyamotoi in …

Evaluation of molecular methods for detection of Borrelia burgdorferi senso lato in ticks

J Yang, Z Liu, G Guan, R Che, Q Niu, Y Li, J Liu… - … and infectious disease, 2012 - Elsevier
Borrelia burgdorferi sensu lato (sl), the agent of Lyme disease, is distributed widely
worldwide. A large number of polymerase chain reaction (PCR) methods have been …

Comparison of Direct Fluorescent Antibody Staining and Real-Time Polymerase Chain Reaction for the Detection of Borrelia Burgdorferi in Ixodes Scapularis Ticks

G Gaumond, A Tyropolis, S Grodzicki… - Journal of veterinary …, 2006 - journals.sagepub.com
Borrelia burgdorferi, the agent responsible for causing Lyme disease in humans and
animals, is transmitted via the bite of infected Ixodes spp. ticks. Ticks removed from humans …

Molecular analysis of Ixodes granulatus, a possible vector tick for Borrelia burgdorferi sensu lato in Taiwan

LL Chao, WJ Wu, CM Shih - Experimental and Applied Acarology, 2009 - Springer
The genetic identity of Ixodes granulatus ticks was determined for the first time in Taiwan.
The phylogenetic relationships were analyzed by comparing the sequences of mitochondrial …

[HTML][HTML] Metagenomic 16S rRNA gene sequencing survey of Borrelia species in Irish samples of Ixodes ricinus ticks

JS Lambert, MJ Cook, JE Healy, R Murtagh… - PLoS …, 2019 - journals.plos.org
The spirochetal bacterium Borrelia miyamotoi is a human pathogen and has been identified
in many countries throughout the world. This study reports for the first time the presence of …

Detection of a Borrelia sp. From Ixodes granulatus Ticks Collected From Rodents in Malaysia

JJ Khoo, SN Ishak, FS Lim… - Journal of medical …, 2018 - academic.oup.com
The Borrelia genus consists of spirochete bacteria known to cause Lyme disease (LD) and
relapsing fever in humans. Borrelia pathogens are commonly transmitted via arthropod …

Approaches for reverse line blot-based detection of microbial pathogens in Ixodes ricinus ticks collected in Austria and impact of the chosen method

AM Schötta, M Wijnveld, H Stockinger… - Applied and …, 2017 - Am Soc Microbiol
Ticks transmit a large number of pathogens capable of causing human disease. In this study,
the PCR-reverse line blot (RLB) method was used to screen for pathogens in a total of 554 …

Development and evaluation of a two-step multiplex TaqMan real-time PCR assay for detection/quantification of different genospecies of Borrelia burgdorferi sensu …

M Nunes, R Parreira, T Carreira, J Inácio… - Ticks and tick-borne …, 2018 - Elsevier
Nowadays, at least four clinically important B. burgdorferi sensu lato (sl) genospecies (B.
afzelii, B. garinii, B. burgdorferi sensu stricto (ss) and B. lusitaniae) circulate in Portugal …

[HTML][HTML] Shifts in Borrelia burgdorferi (sl) geno-species infections in Ixodes ricinus over a 10-year surveillance period in the city of Hanover (Germany) and Borrelia …

K Blazejak, MK Raulf, E Janecek, D Jordan… - Parasites & vectors, 2018 - Springer
Background Lyme borreliosis caused by spirochetes of the Borrelia burgdorferi (sensu lato)
complex is still the most common tick-borne disease in Europe, posing a considerable threat …