In order to understand how β-(1→ 4)-galactosyltransferase recognizes its glycosyl acceptor,
substrate specificities were investigated using synthetic 2-acetamido-2-deoxy-d …

N-Acetyllactosamine is the most prevalent disaccharide moiety in the glycans on the surface
of mammalian cells and often found as repeat units in the linear and branched …

Abstract α1, 3galactosyltransferase (α1, 3GalT) catalyzes the synthesis of a range of
glycoconjugates containing the Galα1, 3Gal epitope which is recognized by the naturally …

Bovine β-(1→ 4)-galactosyltransferase was assayed with a series of 5a-carba-sugars, ie,
sugar analogues in which the ring oxygen of pyranose is replaced by a methylene group …

The capacity of UDP-N-acetyl-α-D-glucosamine (UDP-GlcNAc) as an in vitro acceptor
substrate for β-1, 4-galactosyltransferase (β4GalT1, EC 2.4. 1.38) from human and bovine …

β1, 4-Galactosyltransferase I (Gal-T1) normally transfers Gal from UDP-Gal to GlcNAc in the
presence of Mn 2+ ion. In the presence of α-lactalbumin (LA), the Gal acceptor specificity is …

A number of N-and C-terminal deletion and point mutants of bovine β-1, 4
galactosyltransferase (β-1, 4GT) were expressed in E. coli to determine the binding regions …

Abstract Unlike classical β-1, 4-galactosyltransferase (β-1, 4-GalT I), β-1, 4-GalT V (formerly
IV*) has little activity towards 1 mM N-acetylglucosamine [Sato et al.(1998) Proc Natl Acad …

We inserted a full‐length murine cDNA, which had been isolated from F9 embryonal
carcinoma cells by using a bovine lactose synthetase A protein cDNA as a probe, in a …

A protease‐defective strain of Saccharomyces cerevisiae (BT 150) was used to express full‐
length cDNA of HeLa cell β‐D‐N‐acetylglucosaminide‐β‐1, 4‐galactosyltransferase (gal‐T) …