Role of a conserved acidic cluster in bovine β1, 4 galactosyltransferase-1 probed by mutagenesis of a bacterially expressed recombinant enzyme
Y Zhang, VA Malinovskii, TJ Fiedler, K Brew - Glycobiology, 1999 - academic.oup.com
The truncated catalytic domain of bovine β1, 4 galactosyltransferase-1 was expressed as
inclusion bodies in E. coli and folded to generate 10–15 mg of active enzyme per liter of …
inclusion bodies in E. coli and folded to generate 10–15 mg of active enzyme per liter of …
Characterization and membrane organization of β1→ 3-and β1→ 4-galactosyltransferases from human colonic adenocarcinoma cell lines Colo 205 and SW403 …
EH Holmes - Archives of biochemistry and biophysics, 1989 - Elsevier
Evidence indicates that activation of a β1→ 3N-acetylglucosaminyltransferase is responsible
for accumulation of large quantities of lacto-series tumor-associated antigens in human …
for accumulation of large quantities of lacto-series tumor-associated antigens in human …
Molecular cloning of a human cDNA encoding β-1, 4-galactosyltransferase with 37% identity to mammalian UDP-Gal: GlcNAc β-1, 4-galactosyltransferase
T Sato, K Furukawa, H Bakker… - Proceedings of the …, 1998 - National Acad Sciences
A cDNA encoding a β-1, 4-galactosyltransferase named β-1, 4-GalT II was cloned from a
cDNA library of the human breast tumor cell line, MRK-nu-1. Initially, a 860-bp PCR fragment …
cDNA library of the human breast tumor cell line, MRK-nu-1. Initially, a 860-bp PCR fragment …
Donor substrate promiscuity of bacterial β1–3-N-acetylglucosaminyltransferases and acceptor substrate flexibility of β1–4-galactosyltransferases
Y Li, M Xue, X Sheng, H Yu, J Zeng, V Thon… - Bioorganic & medicinal …, 2016 - Elsevier
Abstract β1–3-N-Acetylglucosaminyltransferases (β3GlcNAcTs) and β1–4-
galactosyltransferases (β4GalTs) have been broadly used in enzymatic synthesis of N …
galactosyltransferases (β4GalTs) have been broadly used in enzymatic synthesis of N …
Identification of a α-NeuAc-(2→ 3)-β-d-galactopyranosyl N-acetyl-β-d-galactosaminyltransferase in human kidney
F Piller, D Blanchard, M Huet, JP Cartron - Carbohydrate research, 1986 - Elsevier
Microsomal preparations from human kidney were found to contain enzymic activity capable
to transfer N-acetylgalactosamine from UDP-N-acetylgalactosamine to native bovine fetuin …
to transfer N-acetylgalactosamine from UDP-N-acetylgalactosamine to native bovine fetuin …
Substrate-assisted and nucleophilically assisted catalysis in bovine α1, 3-galactosyltransferase. Mechanistic implications for retaining glycosyltransferases
H Gomez, JM Lluch, L Masgrau - Journal of the American …, 2013 - ACS Publications
Glycosyltransferases (GTs) are responsible for the biosynthesis of glycans, the most
abundant organic molecules in nature. Their biological relevance makes necessary the …
abundant organic molecules in nature. Their biological relevance makes necessary the …
Oligosaccharide preferences of β1, 4-galactosyltransferase-I: Crystal structures of Met340His mutant of human β1, 4-galactosyltransferase-I with a pentasaccharide …
V Ramasamy, B Ramakrishnan, E Boeggeman… - Journal of molecular …, 2005 - Elsevier
β-1, 4-Galactosyltransferase-I (β4Gal-T1) transfers galactose from UDP-galactose to N-
acetylglucosamine (GlcNAc) residues of the branched N-linked oligosaccharide chains of …
acetylglucosamine (GlcNAc) residues of the branched N-linked oligosaccharide chains of …
Active site studies of bovine α1→ 3-galactosyltransferase and its secondary structure prediction
The catalytic domain of bovine α1→ 3-galactosyltransferase (α3GalT), residues 80–368,
have been cloned and expressed, in Escherichia coli. Using a sequential purification …
have been cloned and expressed, in Escherichia coli. Using a sequential purification …
[HTML][HTML] The acceptor substrate specificity of human β4-galactosyltransferase V indicates its potential function in O-glycosylation
I Van Die, A Van Tetering, WECM Schiphorst, T Sato… - FEBS letters, 1999 - Elsevier
In order to assess the function of the different human UDP-Gal: GlcNAc β4-
galactosyltransferases, the cDNAs of two of them, β4-GalT I and β4-GalT V, were expressed …
galactosyltransferases, the cDNAs of two of them, β4-GalT I and β4-GalT V, were expressed …
Site‐specific linking of biomolecules via glycan residues using glycosyltransferases
PK Qasba, E Boeggeman… - Biotechnology …, 2008 - Wiley Online Library
The structural information on glycosyltransferases has revealed that the sugar‐donor
specificity of these enzymes can be broadened to include modified sugars with a chemical …
specificity of these enzymes can be broadened to include modified sugars with a chemical …