Glycosyltransferases (GTs) catalyze the formation of regio-and stereo-specific glycosidic
linkages between specific sugar donors and recipients. In this study, the function of the gene …

Several studies showed that Sf-9 cells can synthesize the galactosylated N-linked
oligosaccharides if β-1, 4-galactosyltransferase (β-1, 4-GalT) is supplied. The full-length …

Abstract Development of tandem mass spectral methods for direct linkage determination in
oligosaccharides requires sets of trisaccharides differing only in one structural parameter. In …

β-d-Galactosidase from Bacillus circulans catalyzed the transfer of galactose from lactose
predominantly to the OH-4 position of, respectively, GlcNAc and GalNAc to afford β-d-Gal …

Two fusion enzymes consisting of uridine diphosphogalactose 4-epimerase (UDP-galactose
4-epimerase, EC 5.1. 3.2) and α1, 3-galactosyltransferase (EC 2.4. 1.151) with an N-terminal …

Abstract The spacer-modified disaccharide 1, 10-di-O-(2-acetamido-2-deoxy-β-d-
glucopyranosyl)-2-azi-1, 10-decanedial (10) that mimics the biantennary core …

BACKGROUND: Modifications of proteins by O-glycosylation determine many of the
properties and functions of proteins. We wish to understand the mechanisms of O …

The kinetic basis of the donor substrate specificity of β1, 4-N-acetylgiucosaminyltrans-ferase
HI (GnT-III) was investigated using a purified recombinant enzyme. The enzyme also …

A molecular docking study has been performed on the interaction of β1, 4-
galactosyltransferase with an acceptor site photoprobe. This is based on an acceptor site …

A lectin-assisted enzyme-linked immunosorbent assay (ELISA)-based method using a
synthetic glycopolymer as an acceptor substrate was developed for measuring β1, 4 …