This review summarizes our recent works concerned on the analysis of glycoprotein
glycans. Glycoprotein glycans are highly complicated and have variations that number into …

Oligosaccharides attached to proteins are very challenging analytical targets because of
their wide variety and complexity. Reliable analytical methods for the quantitative profiling of …

Many important biological processes are mediated by the carbohydrate moieties of
glycoconjugates. Their structural determination presents considerable difficulties because of …

Monitoring protein glycosylation is becoming increasingly important as novel recombinant
glycoprotein therapeutics, such as glycoprotein hormones, cytokines and clotting factors, are …

N-glycosylation is one of the critical quality attributes for the therapeutic monoclonal
antibodies. Characterization of N-glycans of monoclonal antibodies provides valuable …

We here present a printed covalent glycan microarray for protein-binding studies, using low-
femtomole quantities of glycans. Glycans, either natural glycans, which were released from …

Efficient enzymatic digestion methods are critical for the characterization and identification of
glycans. Glycan hydrolysis enzymes are widely utilized for the identification of glycoprotein …

We report rapid and efficient electrophoretic separations of N-glycans on microfluidic
devices. Using a separation length of 22 cm and an electric field strength of 750 V/cm …

N-linked glycans harbored on glycoproteins profoundly affect the character of proteins by
altering their structure or capacity to bind to other molecules. Specific knowledge of the role …

Proteoglycans are key biological macromolecules that, via their glycan constituents,
participate and regulate several cellular events and physiopathological processes. Refined …