Role of a conserved acidic cluster in bovine β1, 4 galactosyltransferase-1 probed by mutagenesis of a bacterially expressed recombinant enzyme
Y Zhang, VA Malinovskii, TJ Fiedler, K Brew - Glycobiology, 1999 - academic.oup.com
The truncated catalytic domain of bovine β1, 4 galactosyltransferase-1 was expressed as
inclusion bodies in E. coli and folded to generate 10–15 mg of active enzyme per liter of …
inclusion bodies in E. coli and folded to generate 10–15 mg of active enzyme per liter of …
The N-acetyl-binding pocket of N-acetylglucosaminyltransferases also accommodates a sugar analog with a chemical handle at C2
M Pasek, B Ramakrishnan, E Boeggeman… - …, 2012 - academic.oup.com
In recent years, sugars with a unique chemical handle have been used to detect and
elucidate the function of glycoconjugates. Such chemical handles have generally been part …
elucidate the function of glycoconjugates. Such chemical handles have generally been part …
On the preparative use of recombinant β (1–3) galactosyl-transferase
G Baisch, R Öhrlein, M Streiff, F Kolbinger - Bioorganic & medicinal …, 1998 - Elsevier
A number of non-natural N-acyl derivatives of glucosamine is incubated with a recombinant
β (1–3) galactosyl-transferase and UDP-galactose. Surprisingly, the enzyme recognizes the …
β (1–3) galactosyl-transferase and UDP-galactose. Surprisingly, the enzyme recognizes the …
Studies on the metal binding sites in the catalytic domain of β1, 4-galactosyltransferase
E Boeggeman, PK Qasba - Glycobiology, 2002 - academic.oup.com
The catalytic domain of bovine β1, 4-galactosyltransferase (β4Gal-T1) has been shown to
have two metal binding sites, each with a distinct binding affinity. Site I binds Mn2+ with high …
have two metal binding sites, each with a distinct binding affinity. Site I binds Mn2+ with high …
In situ generated O-Glycan core 1 structure as substrate for Gal (β1–3) GalNAc β-1, 6-GlcNAc Transferase
G Dudziak, S Zeng, EG Berger, RG Gallego… - Bioorganic & medicinal …, 1998 - Elsevier
β-Galactosidase from bovine testes was used in a one pot reaction together with a
recombinant β-1, 6-GlcNAc transferase for the synthesis of GlcNAc (β1–6) GalNAc (α1 …
recombinant β-1, 6-GlcNAc transferase for the synthesis of GlcNAc (β1–6) GalNAc (α1 …
A novel human β1, 3-N-acetylgalactosaminyltransferase that synthesizes a unique carbohydrate structure, GalNAcβ1-3GlcNAc
T Hiruma, A Togayachi, K Okamura, T Sato… - Journal of Biological …, 2004 - ASBMB
We found, using a BLAST search, a novel human gene (GenBank™ accession number
BC029564) that possesses β3-glycosyltransferase motifs. The full-length open reading …
BC029564) that possesses β3-glycosyltransferase motifs. The full-length open reading …
Direct identification of nonreducing GlcNAc residues on N-glycans of glycoproteins using a novel chemoenzymatic method
E Boeggeman, B Ramakrishnan, C Kilgore… - Bioconjugate …, 2007 - ACS Publications
The mutant β1, 4-galactosyltransferase (β4Gal-T1), β4Gal-T1-Y289L, in contrast to wild-type
β4Gal-T1, can transfer GalNAc from the sugar donor UDP-GalNAc to the acceptor, GlcNAc …
β4Gal-T1, can transfer GalNAc from the sugar donor UDP-GalNAc to the acceptor, GlcNAc …
A Fluorescent Assay for the Determination of UDP-GlcNAc: Galβ1, 3GalNAc-R (GlcNAc to GalNAc) β-1, 6 N-Acetylglucosaminyltransferase Activity
CA Palmerini, A Datti, S Alunni, IE VanderElst… - Analytical …, 1995 - Elsevier
A fluorescent assay for UDP-GlcNAc: Galβ1, 3GalNAc-R β1, 6-N-
acetylglucosaminyltransferase (core 2 GlcNAc-T) activity has been developed involving …
acetylglucosaminyltransferase (core 2 GlcNAc-T) activity has been developed involving …
Biosynthesis of the linkage region of glycosaminoglycans: cloning and activity of galactosyltransferase II, the sixth member of the β1, 3-galactosyltransferase family …
X Bai, D Zhou, JR Brown, BE Crawford… - Journal of Biological …, 2001 - ASBMB
A family of five β1, 3-galactosyltransferases has been characterized that catalyze the
formation of Galβ1, 3GlcNAcβ and Galβ1, 3GalNAcβ linkages present in glycoproteins and …
formation of Galβ1, 3GlcNAcβ and Galβ1, 3GalNAcβ linkages present in glycoproteins and …
Production and characterization of active soluble human β1, 4-galactosyltransferase in Escherichia coli as a useful catalyst in synthesis of the Gal β1→ 4 GlcNAc …
S Shibatani, K Fujiyama, S Nishiguchi, T Seki… - Journal of bioscience …, 2001 - Elsevier
An active and soluble human β1, 4-galactosyltransferase (β-GT) was produced in
Escherichia coli using a maltose-binding protein fusion system. The purified recombinant β …
Escherichia coli using a maltose-binding protein fusion system. The purified recombinant β …