DNA binding specificity of the CCAAT-binding factor CBF/NF-Y

W Bi, L Wu, F Coustry, B De Crombrugghe… - Journal of Biological …, 1997 - ASBMB
CBF is a heterotrimeric protein that binds to DNA containing CCAAT motifs. Here we have
analyzed interactions of recombinant CBF with DNA using hydroxyl radical footprinting and …

Recombinant rat CBF-C, the third subunit of CBF/NFY, allows formation of a protein-DNA complex with CBF-A and CBF-B and with yeast HAP2 and HAP3.

S Sinha, SN Maity, J Lu… - Proceedings of the …, 1995 - National Acad Sciences
The CCAAT binding factor CBF is a heteromeric transcription factor, which binds to
functional CCAAT motifs in many eukaryotic promoters. cDNAs for the A and B subunits of …

Studies on Transcription Activation by the Multimeric CCAAT-binding Factor CBF (∗)

F Coustry, SN Maity, B de Crombrugghe - Journal of Biological Chemistry, 1995 - ASBMB
The CCAAT-binding factor CBF is a heteromeric transcription factor that specifically binds to
CCAAT sequences in many eukaryotic genes. CBF consists of three subunits, CBF-A, CBF …

Role of the CCAAT-binding protein CBF/NF-Y in transcription

SN Maity, B De Crombrugghe - Trends in biochemical sciences, 1998 - cell.com
The CCAAT motif is one of the common promoter elements present in the proximal promoter
of numerous mammalian genes transcribed by RNA polymerase II. CBF (also called NF-Y …

Determination of functional domains in the C subunit of the CCAAT-binding factor (CBF) necessary for formation of a CBF-DNA complex: CBF-B interacts …

IS Kim, S Sinha, B De Crombrugghe… - Molecular and cellular …, 1996 - Am Soc Microbiol
The mammalian CCAAT-binding factor (CBF; also called NF-Y and CP1) is a heterotrimeric
protein consisting of three subunits, CBF-A, CBF-B, and CBF-C, all of which are required for …

Stable expression of a dominant negative mutant of CCAAT binding factor/NF-Y in mouse fibroblast cells resulting in retardation of cell growth and inhibition of …

Q Hu, SN Maity - Journal of Biological Chemistry, 2000 - ASBMB
The heterotrimeric CCAAT-binding factor CBF specifically interacts with the CCAAT motif
present in the proximal promoters of numerous mammalian genes. To understand the in vivo …

Three classes of mutations in the A subunit of the CCAAT-binding factor CBF delineate functional domains involved in the three-step assembly of the CBF-DNA …

S Sinha, IS Kim, KY Sohn… - … and cellular biology, 1996 - Am Soc Microbiol
The mammalian CCAAT-binding factor CBF (also called NF-Y or CP1) consists of three
subunits, CBF-A, CBF-B, and CBF-C, all of which are required for DNA binding and present …

The transcriptional activity of the CCAAT-binding factor CBF is mediated by two distinct activation domains, one in the CBF-B subunit and the other in the CBF-C …

F Coustry, SN Maity, S Sinha… - Journal of Biological …, 1996 - ASBMB
CBF-A, CBF-B, and CBF-C together form the heterotrimeric mammalian CCAAT-binding
factor, CBF, which binds to DNA to form a CBF-DNA complex. Here we examined the …

The molecular biology of the CCAAT-binding factor NF-Y

R Mantovani - Gene, 1999 - Elsevier
Protein coding genes are transcribed by Polymerase II, under the control of short discrete
DNA elements in promoters and enhancers, recognized with high efficiency and specificity …

Human p32, interacts with B subunit of the CCAAT-binding factor, CBF/NF-Y, and inhibits CBF-mediated transcription activation in vitro

C Chattopadhyay, D Hawke, R Kobayashi… - Nucleic acids …, 2004 - academic.oup.com
To understand the role of the CCAAT-binding factor, CBF, in transcription, we developed a
strategy to purify the heterotrimeric CBF complex from HeLa cell extracts using two …