A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans

MK Chaveroche, JM Ghigo, C d'Enfert - Nucleic acids research, 2000 - academic.oup.com
… In this report, we have presented a rapid, simple and efficient methodology to generate allelic
replacements in the filamentous fungus A.nidulans. This methodology takes advantage of …

Efficient PCR-based gene targeting with a recyclable marker for Aspergillus nidulans

ML Nielsen, L Albertsen, G Lettier, JB Nielsen… - Fungal Genetics and …, 2006 - Elsevier
gene-targeting techniques in filamentous fungi and other higher eukaryotes has been
hampered because foreign DNA … sequence and efficient gene-targeting methods has made this …

A Versatile and Efficient Gene-Targeting System for Aspergillus nidulans

T Nayak, E Szewczyk, CE Oakley, A Osmani, L Ukil… - Genetics, 2006 - academic.oup.com
… The filamentous fungus Aspergillus nidulans is an important experimental organism. … a
highly efficient gene targeting for A. nidulans that uses nkuAΔ, a deletion of a gene required for …

Transient disruption of non-homologous end-joining facilitates targeted genome manipulations in the filamentous fungus Aspergillus nidulans

JB Nielsen, ML Nielsen, UH Mortensen - Fungal Genetics and Biology, 2008 - Elsevier
… We have developed a transiently disrupted nkuA system in Aspergillus nidulans for efficient
gene targeting. The nkuA disruption was made by inserting a counter-selectable marker …

[PDF][PDF] Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans

MJ Daboussi, A Djeballi, C Gerlinger… - Current …, 1989 - researchgate.net
… A gene transfer system originally developed for Fusarium oxysporum has been applied
to seven species of filamentous fungi of agricultural and industrial importance. This …

A novel improved method for Aspergillus nidulans transformation

M Koukaki, E Giannoutsou, A Karagouni… - … microbiological methods, 2003 - Elsevier
genetic approaches to filamentous fungi has been achieved since the development of
transformation protocols for Aspergillus nidulans … It appears possible that all fungal species that …

Direct and Indirect Gene Replacements in Aspergillus nidulans

BL Miller, KY Miller, WE Timberlake - Molecular and cellular …, 1985 - Taylor & Francis
… approaches afforded by the yeast gene replacement technology to answer questions about
… for integrative transformation of the filamentous fungus Aspergillus nidulans, a relative of S.…

A rapid method for promoter exchange in Aspergillus nidulans using recombinant PCR

M Zarrin, AC Leeder, G Turner - Fungal Genetics and Biology, 2005 - Elsevier
… (http://www.broad.mit.edu/annotation/fungi/aspergillus/ ), one of the major genetic model …
for filamentous fungi, has led to an urgent need to develop rapid techniques for analysis of gene

[HTML][HTML] Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi

RJN Frandsen, JA Andersson, MB Kristensen… - BMC Molecular …, 2008 - Springer
… , we constructed a variant of the pAg1-H3 vector [5], in which the gpdA promoter from
Aspergillus nidulans was inserted pointing towards the left border (LB) sequence (Figure 3). The A. …

[HTML][HTML] Methods for genetic transformation of filamentous fungi

D Li, Y Tang, J Lin, W Cai - Microbial cell factories, 2017 - Springer
Aspergillus nidulans has been one of the most widely studied species in terms of
genetics and biochemistry [9, 10]. It is often used as model organism in the identification of …