An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency
D Gems, IL Johnstone, AJ Clutterbuck - Gene, 1991 - Elsevier
… sequence, ansl, which increases transformation efficiency considerably, apparently by
stimulating plasmid integration. Among filamentous fungi only from the Zygomycete Mucor …
stimulating plasmid integration. Among filamentous fungi only from the Zygomycete Mucor …
… free gene replacement in citric acid-producing Aspergillus tubingensis (A. niger) WU-2223L by the CRISPR/Cas9 system-based genome editing technique using DNA …
I Yoshioka, K Kirimura - Journal of bioscience and bioengineering, 2021 - Elsevier
… enable rapid and efficient gene replacement in citric acid-producing fungi for genetic breeding.
… via fermentation using filamentous fungi belonging to Aspergillus section Nigri, such as …
… via fermentation using filamentous fungi belonging to Aspergillus section Nigri, such as …
Engineering of filamentous fungi for efficient conversion of lignocellulose: tools, recent advances and prospects
G Liu, Y Qu - Biotechnology Advances, 2019 - Elsevier
… Recently, the development of efficient genetic manipulation systems in several … Here, we
review the recent advances in the engineering of filamentous fungi for efficient lignocellulose …
review the recent advances in the engineering of filamentous fungi for efficient lignocellulose …
Genetic Manipulation and Transformation Methods for Aspergillus spp.
… tumefaciens is used as a vector for gene transfer in various filamentous fungi. AMT was first
… available gene editing methods and transformation efficiency are low, but filamentous fungi …
… available gene editing methods and transformation efficiency are low, but filamentous fungi …
New tools for the genetic manipulation of filamentous fungi
… , which is required for proper conidiophore development in Aspergillus nidulans. Upon
induction, RNAi strains appear colorless because of the loss of their green conidiospores. …
induction, RNAi strains appear colorless because of the loss of their green conidiospores. …
High‐Throughput Gene Replacement in Aspergillus fumigatus
C Zhao, MG Fraczek, L Dineen… - Current Protocols in …, 2019 - Wiley Online Library
… species, such as Aspergillus niger or Aspergillus nidulans. … high-efficiency homologous
recombination in filamentous fungi. … , effective gene replacement requires the generation of gene …
recombination in filamentous fungi. … , effective gene replacement requires the generation of gene …
Stepwise genetic modification for efficient expression of heterologous proteins in Aspergillus nidulans
Q Yan, L Han, Z Liu, S Zhou, Z Zhou - Applied Microbiology and …, 2023 - Springer
… Aspergillus nidulans is an important model strain of filamentous fungi, but not a fully … However,
contrary to the ongoing development of efficient gene expression systems, weak synthesis …
contrary to the ongoing development of efficient gene expression systems, weak synthesis …
Highly efficient gene targeting in the Aspergillus niger kusA mutant
V Meyer, M Arentshorst, A El-Ghezal, AC Drews… - Journal of …, 2007 - Elsevier
… genomes requires the development of efficient tools for the functional characterization of
genes in filamentous fungi, for example by an efficient method to construct gene knock-outs. …
genes in filamentous fungi, for example by an efficient method to construct gene knock-outs. …
Genetic transformation in Aspergilli: tools of the trade
VL Prabha, NS Punekar - 2004 - nopr.niscpr.res.in
… In filamentous fungi, genetic transformation is usually achieved by integration of the introduced
DNA into … An efficient genetic transformation system is really a prerequisite for functional …
DNA into … An efficient genetic transformation system is really a prerequisite for functional …
Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus
C Zhang, X Meng, X Wei, L Lu - Fungal Genetics and Biology, 2016 - Elsevier
… In addition, in contrast to the model species Aspergillus nidulans (A. … is not particularly
efficient. Because filamentous fungi have a dominant NHEJ DNA repair pathway that competes …
efficient. Because filamentous fungi have a dominant NHEJ DNA repair pathway that competes …