An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency

D Gems, IL Johnstone, AJ Clutterbuck - Gene, 1991 - Elsevier
… sequence, ansl, which increases transformation efficiency considerably, apparently by
stimulating plasmid integration. Among filamentous fungi only from the Zygomycete Mucor …

… free gene replacement in citric acid-producing Aspergillus tubingensis (A. niger) WU-2223L by the CRISPR/Cas9 system-based genome editing technique using DNA …

I Yoshioka, K Kirimura - Journal of bioscience and bioengineering, 2021 - Elsevier
… enable rapid and efficient gene replacement in citric acid-producing fungi for genetic breeding.
… via fermentation using filamentous fungi belonging to Aspergillus section Nigri, such as …

Engineering of filamentous fungi for efficient conversion of lignocellulose: tools, recent advances and prospects

G Liu, Y Qu - Biotechnology Advances, 2019 - Elsevier
… Recently, the development of efficient genetic manipulation systems in several … Here, we
review the recent advances in the engineering of filamentous fungi for efficient lignocellulose …

Genetic Manipulation and Transformation Methods for Aspergillus spp.

YE Son, HS Park - Mycobiology, 2021 - Taylor & Francis
… tumefaciens is used as a vector for gene transfer in various filamentous fungi. AMT was first
… available gene editing methods and transformation efficiency are low, but filamentous fungi

New tools for the genetic manipulation of filamentous fungi

U Kück, B Hoff - Applied microbiology and biotechnology, 2010 - Springer
… , which is required for proper conidiophore development in Aspergillus nidulans. Upon
induction, RNAi strains appear colorless because of the loss of their green conidiospores. …

High‐Throughput Gene Replacement in Aspergillus fumigatus

C Zhao, MG Fraczek, L Dineen… - Current Protocols in …, 2019 - Wiley Online Library
… species, such as Aspergillus niger or Aspergillus nidulans. … high-efficiency homologous
recombination in filamentous fungi. … , effective gene replacement requires the generation of gene

Stepwise genetic modification for efficient expression of heterologous proteins in Aspergillus nidulans

Q Yan, L Han, Z Liu, S Zhou, Z Zhou - Applied Microbiology and …, 2023 - Springer
Aspergillus nidulans is an important model strain of filamentous fungi, but not a fully … However,
contrary to the ongoing development of efficient gene expression systems, weak synthesis …

Highly efficient gene targeting in the Aspergillus niger kusA mutant

V Meyer, M Arentshorst, A El-Ghezal, AC Drews… - Journal of …, 2007 - Elsevier
… genomes requires the development of efficient tools for the functional characterization of
genes in filamentous fungi, for example by an efficient method to construct gene knock-outs. …

Genetic transformation in Aspergilli: tools of the trade

VL Prabha, NS Punekar - 2004 - nopr.niscpr.res.in
… In filamentous fungi, genetic transformation is usually achieved by integration of the introduced
DNA into … An efficient genetic transformation system is really a prerequisite for functional …

Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus

C Zhang, X Meng, X Wei, L Lu - Fungal Genetics and Biology, 2016 - Elsevier
… In addition, in contrast to the model species Aspergillus nidulans (A. … is not particularly
efficient. Because filamentous fungi have a dominant NHEJ DNA repair pathway that competes …