Using Aspergillus nidulans to identify antifungal drug resistance mutations

X He, S Li, SGW Kaminskyj - Eukaryotic cell, 2014 - Am Soc Microbiol
… Identification of genes whose mutations lead to targeted resistance can provide new
information on those pathways. We used Aspergillus nidulans as a model system to exploit its …

Elimination of marker genes from transformed filamentous fungi by unselected transient transfection with a Cre-expressing plasmid

S Florea, K Andreeva, C Machado, PM Mirabito… - Fungal Genetics and …, 2009 - Elsevier
… in many filamentous fungi, gene replacement is routinely performed for elucidation of gene
function in … coenophialum, two other epichloae, and the genetic model, Aspergillus nidulans. …

Heterologous gene expression in filamentous fungi

X Su, G Schmitz, M Zhang, RI Mackie… - Advances in applied …, 2012 - Elsevier
… in using filamentous fungi (particularly Aspergillus spp., … techniques commonly employed
in recombinant protein production in the filamentous fungi, including transformation methods

Strain improvement in filamentous fungi-an overview

KMH Nevalainen - Applied mycology and biotechnology, 2001 - Elsevier
… transformation and protein engineering are being applied for the further refinement of fungal
… of gene products and of secreting them into the culture medium, filamentous fungi are …

Development of a new Agrobacterium-mediated transformation system based on a dual auxotrophic approach in the filamentous fungus Aspergillus oryzae

HD Thai, BPT Nguyen, VM Nguyen, QH Nguyen… - World Journal of …, 2021 - Springer
… We further established a new ATMT system based on the dual auxotrophic strains with the
use of hisB and pyrG as the selectable markers for efficient genetic manipulations in A. oryzae…

Advances in Genetic Engineering Technology and Its Application in the Industrial Fungus Aspergillus oryzae

FJ Jin, S Hu, BT Wang, L Jin - Frontiers in Microbiology, 2021 - frontiersin.org
… The filamentous fungus Aspergillus oryzae is an important strain in the traditional … efficient
genetic transformation system is a prerequisite for genetic manipulation and recombinant gene

Expanding the ku70 toolbox for filamentous fungi: establishment of complementation vectors and recipient strains for advanced gene analyses

NDSP Carvalho, M Arentshorst, M Jin Kwon… - Applied microbiology …, 2010 - Springer
… The autonomous maintenance in A spergillus (AMA1) sequence, isolated from a genomic
library of Aspergillus nidulans, is based on two inverted sequences surrounding a unique …

Agrobacterium tumefaciens-mediated transformation of NHEJ mutant aspergillus nidulans conidia: an efficient tool for targeted gene recombination using selectable …

V Casado-del Castillo, AP MacCabe, M Orejas - Journal of Fungi, 2021 - mdpi.com
… , is still widely used especially in the model filamentous fungi-including Aspergillus nidulans
[3]. Empirical modifications of the technique have, in general, either sought to improve …

Development of versatile and efficient genetic tools for the marine-derived fungus Aspergillus terreus RA2905

G Yao, X Chen, Y Han, H Zheng, Z Wang, J Chen - Current Genetics, 2022 - Springer
… further improve gene deletion efficiency. In conclusion, efficient genetic manipulation systems
… (hph), are commonly used as selectable markers in model filamentous fungi. Besides, …

Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi

JH Yu, Z Hamari, KH Han, JA Seo… - Fungal genetics and …, 2004 - Elsevier
nidulans, Aspergillus fumigatus, and Fusarium graminearum. In addition, simple and fast
methods to isolate filamentous fungal genomic DNA … marker in Aspergillus nidulans. Eukaryot. …