Intranuclear distribution of U1 and U2 snRNAs visualized by high resolution in situ hybridization: revelation of a novel compartment containing U1 but not U2 snRNA in …

N Visa, F Puvion-Dutilleul, JP Bachellerie… - European journal of …, 1993 - europepmc.org
… We have examined the intranuclear distribution of U1 and U2 small nuclear RNAs (snRNAs)
in HeLa cells by electron microscope in situ hybridization using biotinylated DNA probes …

Transcription-dependent colocalization of the U1, U2, U4/U6, and U5 snRNPs in coiled bodies

M Carmo-Fonseca, R Pepperkok, MT Carvalho… - The Journal of cell …, 1992 - rupress.org
… In situ hybridization with Ul (G), U2 (N), and U3 (I) snRNA probes … distribution ofnuclear
ribonucleoproteins as visualized by … factor with a novel intranuclear distribution. EMBO (Eur. Mol. …

U2 and U1 snRNA gene loci associate with coiled bodies

KP Smith, KC Carter, CV Johnson… - Journal of cellular …, 1995 - Wiley Online Library
… Using fluorescence in situ hybridization, we report here the … series 200 CCD camera using
a high resolution, shallow depth-of-… with a novel intranuclear distribution. EMBO J 10:207-214. …

[图书][B] Spliceosomal small nuclear ribonucleoprotein particle trafficking and maturation in the nuclear compartment

SB Patel - 2010 - search.proquest.com
… 15 In this study, nascent U2 snRNA was detected with RNA … by in situ hybridization that
more than 90% of the nuclear U7 … region of U1 snRNA is critical for its intranuclear trafficking, it …

[图书][B] Nucleolar localization and maintenance of U3 small nucleolar RNA in Xenopus laevis

R Rivera-Leon - 1995 - search.proquest.com
Higher quality 6” x 9" black and white photographic prints are … It includes protocols for cell
transfections, in situ hybridizations… in spliceosomal and nucleolar snRNAs are also very similar. …

snRNA-specific role of SMN in trypanosome snRNP biogenesis in vivo

N Jaé, C Preußer, T Krüger, ID Tkacz, M Engstler… - RNA biology, 2011 - Taylor & Francis
snRNP biogenesis, fluorescence in situ hybridization (FISH) … , in that it remains intranuclear
during its maturation, but also … extent the SL RNA and the snRNAs U1, U2, U4, U5 and U6 …

Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts

SB Patel, N Novikova, M Bellini - The Journal of Cell Biology, 2007 - rupress.org
… In particular, we demonstrate that U1 and U2 snRNPs need … by in situ hybridization that
>90% of the nuclear U7 snRNA … region of U1 snRNA is critical for its intranuclear trafficking, it …

[HTML][HTML] Modification of Sm small nuclear RNAs occurs in the nucleoplasmic Cajal body following import from the cytoplasm

BE Jády, X Darzacq, KE Tucker, AG Matera… - The EMBO …, 2003 - embopress.org
… modification of the U2 snRNA occurs exclusively after … and U5 RNAs was detected by in situ
hybridization with … pseudouridylation of the U1, U2, U4 and U5 Sm snRNAs are directed by …

Splicing-independent recruitment of U1 snRNP to a transcription unit in living cells

B Spiluttini, B Gu, P Belagal… - Journal of cell …, 2010 - journals.biologists.com
… evaluated using fluorescence in situ hybridization (FISH) and … To visualize transcription start
sites, the doxycycline-induced … different colors for U1 snRNA, U2 snRNA or MS2 sequences. …

Intranuclear distribution of poly (A) RNA determined by electron microscope in situ hybridization

N Visa, F Puvion-Dutilleul, F Harper… - Experimental cell …, 1993 - Elsevier
… recently identified which contains U1 but not U2 snRNA. [17]. … [15], snRNP antigens [15, 16],
and U1 and U2 snRNAs [17] further … of snRNP antigens on nascent transcripts visualized on …