Modular (de) construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing
… (de)construction of complex bacterial phenotypes. In this context, we define deconstruction
as … construction is presented as the one-step multiplex editing of gene targets that lead to the …
as … construction is presented as the one-step multiplex editing of gene targets that lead to the …
Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis
D Liu, C Huang, J Guo, P Zhang, T Chen… - Biotechnology for …, 2019 - Springer
… added advantage of modular construction protocols, reduced … All bacterial strains and plasmids
used in this work are listed … The mutations were confirmed by phenotypic validation, PCR …
used in this work are listed … The mutations were confirmed by phenotypic validation, PCR …
[HTML][HTML] Emergent CRISPR–Cas-based technologies for engineering non-model bacteria
… demonstrated multiplexed genome insertions also in the context of a complex bacterial
community … studies or targeted engineering in complex communities. The possibility of modifying …
community … studies or targeted engineering in complex communities. The possibility of modifying …
Systems analysis of highly multiplexed CRISPR-base editing in streptomycetes
… multiplexed base editing, and to the best of our knowledge, 17 targets is the highest number
of base edited genomic loci using Csy4 based multiplexing in bacteria … different phenotypes …
of base edited genomic loci using Csy4 based multiplexing in bacteria … different phenotypes …
Precise genome engineering in Pseudomonas using phage-encoded homologous recombination and the Cascade–Cas3 system
W Zheng, Y Xia, X Wang, S Gao, D Zhou… - Nature …, 2023 - nature.com
… putida 56 ; the multiplexed cytidine base editors technology … , thereby providing modular
cassettes configured for utility … applied for constructing plant growth-promoting bacteria chassis …
cassettes configured for utility … applied for constructing plant growth-promoting bacteria chassis …
An expanded CRISPR–Cas9-assisted recombineering toolkit for engineering genetically intractable Pseudomonas aeruginosa isolates
D Pankratz, NO Gomez, A Nielsen, A Mustafayeva… - Nature protocols, 2023 - nature.com
… The remarkable metabolic versatility and phenotypic … with a variety of antibiotic resistance
modules, enabling the Ssr-… generation of targeted and multiplexed nucleotide substitutions with …
modules, enabling the Ssr-… generation of targeted and multiplexed nucleotide substitutions with …
CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications
… the design and multiplexing of complex genetic functions. … engineering of genotype–phenotype
relationships and genetically … microbial consortia of multiple bioproduction modules (387–…
relationships and genetically … microbial consortia of multiple bioproduction modules (387–…
Towards next-generation cell factories by rational genome-scale engineering
… Moreover, phenotypic improvements of metabolic networks … can also be referred to as
multiplexing, but we will not use this … in bacterial hosts, similar oligo-based multiplex genome …
multiplexing, but we will not use this … in bacterial hosts, similar oligo-based multiplex genome …
[HTML][HTML] Highly efficient multiplex base editing: One-shot deactivation of eight genes in Shewanella oneidensis MR-1
Y Chen, M Cheng, Y Li, L Wang, L Fang, Y Cao… - Synthetic and Systems …, 2023 - Elsevier
… of the multiplex engineered strain HRF(8BE) in microbial fuel … This highly efficient multiplexed
base editing tool elevates … reveal the association between genotype and phenotype [7]. To …
base editing tool elevates … reveal the association between genotype and phenotype [7]. To …
Expanding the flexibility of base editing for high-throughput genetic screens in bacteria
S Gawlitt, SP Collins, Y Yu, SA Blackman… - Nucleic Acids …, 2024 - academic.oup.com
… genotype-to-phenotype relationships in bacteria. Of the … of highthroughput genetic screening
in bacteria. We first co-opt … programmable, targeted and multiplexed gene perturbations as …
in bacteria. We first co-opt … programmable, targeted and multiplexed gene perturbations as …