A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functions
LPG Oliveira, FL Conte, E de Oliveira Cardoso… - Life sciences, 2019 - Elsevier
Life sciences, 2019•Elsevier
Aims Chemotherapy has been widely used to treat cancer although it may affect non-target
cells involved in the immune response. This work aimed at elucidating whether the
chemotherapeutic agent doxorubicin in combination with geopropolis produced by Melipona
fasciculata Smith could affect nontumor immune cells, evaluating their immunomodulatory
effects on human monocytes. Main methods Cell viability, expression of cell markers (HLA-
DR, TLR-2, TLR-4, C80 and CD40), cytokine production (TNF-α, IL-1β, IL-6 and IL-10) …
cells involved in the immune response. This work aimed at elucidating whether the
chemotherapeutic agent doxorubicin in combination with geopropolis produced by Melipona
fasciculata Smith could affect nontumor immune cells, evaluating their immunomodulatory
effects on human monocytes. Main methods Cell viability, expression of cell markers (HLA-
DR, TLR-2, TLR-4, C80 and CD40), cytokine production (TNF-α, IL-1β, IL-6 and IL-10) …
Aims
Chemotherapy has been widely used to treat cancer although it may affect non-target cells involved in the immune response. This work aimed at elucidating whether the chemotherapeutic agent doxorubicin in combination with geopropolis produced by Melipona fasciculata Smith could affect nontumor immune cells, evaluating their immunomodulatory effects on human monocytes.
Main methods
Cell viability, expression of cell markers (HLA-DR, TLR-2, TLR-4, C80 and CD40), cytokine production (TNF-α, IL-1β, IL-6 and IL-10), intracellular pathways (NF-κB and autophagy), the microbicidal activity of monocytes and hydrogen peroxide (H2O2) production were analyzed.
Key findings
Data showed that doxorubicin + geopropolis diminished IL-6 secretion, stimulated TNF-α and IL-10 production, TLR-4 and CD80 expression, NF-κB and autophagy pathway, as well as the bactericidal activity.
Significance
Our findings revealed a new chemotherapeutic approach using doxorubicin simultaneously with geopropolis without affecting human monocytes viability and exerting immunomodulatory effects, favoring cell functions. While doxorubicin altered some immunological parameters, the addition of geopropolis compensated some changes.
Elsevier
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