Fluorescence lifetime measurements are becoming increasingly important in chemical and
biological research. Time-domain lifetime measurements offer fluorescence multiplexing
and improved handling of interferers compared with the frequency-domain technique. In this
paper, an all solid-state, filterless, and highly portable light-emitting-diode based time-
domain fluorimeter (LED TDF) is reported for the measurement of nanosecond fluorescence
lifetimes. LED based excitation provides more wavelengths options compared to laser diode …