Background: Diabetes mellitus has become a global concern with cases that increase every year and contribute to various organ damage. Previous research shows that the butterfly pea flower (Clitoria ternatea) has the potential to lower blood glucose level and protect the pancreas through its antioxidant and antihyperglycemic effects.

Methods: An experimental analytic study was conducted using 15 mice which were divided into 5 groups, N (normal), HG (hyperglycemic without CTE), HG+ MET (hyperglycemic+ metformin), HG+ CTE300 (hyperglycemic+ CTE300mg/kgBB), and HG+ CTE500 (hyperglycemic+ CTE500mg/kgBB). CTE administration was carried out for 2 weeks in groups HG+ CTE300 and HG+ CTE500 which were then examined for blood glucose level and histological features of pancreatic β cells preparation with hematoxylin-eosin staining.

Results: The results of the data analysis showed that there was a significant difference in mean blood glucose level between the HG group and the treatment group with CTE administration (HG+ CTE300 and HG+ CTE500)((MD: 53.67 95%; p= 0.001) and (MD: 57.67 95%; p= 0.001) respectively). In addition, a significant difference was found in the average number of pancreatic β cells per area between the HG group and the treatment group given CTE 500 mg/kg BW (MD:-8.83; p= 0.012). There was no significant difference between the mean blood glucose and the number of pancreatic β cells per area between the groups with CTE compared to the metformin group.

Conclusion: Aqueous extract of butterfly pea flower (Clitoria teratea) has potential effects on lowering blood glucose levels and improving pancreatic β cell features in hyperglycemic mice.