RNA-seq based transcriptomic analysis of single bacterial cells

J Wang, L Chen, Z Chen, W Zhang - Integrative Biology, 2015 - academic.oup.com
… the transcripts in single bacterial cells. In addition, the preliminary results from nitrogen-starved
cells also showed a possible increasing gene-expression heterogeneity from 24 h to 72 h …

New RNA-seq approaches for the study of bacterial pathogens

AE Saliba, SC Santos, J Vogel - Current opinion in microbiology, 2017 - Elsevier
… -called RNA-seq technique whereby transcripts are converted to cDNA for next-generation
sequencing it is now possible to profile … Unsurprisingly, RNA-seq has also become a standard …

Revealing of Mycobacterium marinum Transcriptome by RNA-seq

S Wang, X Dong, Y Zhu, C Wang, G Sun, T Luo… - PloS one, 2013 - journals.plos.org
… , RNA-seq is suited to the application of detecting transcripts which correspond to existing
genomic sequence. Vera JC, et al have used RNA-seq to revealRNA seq, we harvest bacteria

A Strand-Specific RNASeq Analysis of the Transcriptome of the Typhoid Bacillus Salmonella Typhi

TT Perkins, RA Kingsley, MC Fookes, PP Gardner… - PLoS …, 2009 - journals.plos.org
… Typhi OmpR regulon and identify novel OmpR regulated transcripts. Thus, ssRNA–seq
provides a novel and powerful approach to the characterization of the bacterial transcriptome. …

Comprehensive analysis of the Corynebacterium glutamicum transcriptome using an improved RNAseq technique

K Pfeifer-Sancar, A Mentz, C Rückert, J Kalinowski - BMC genomics, 2013 - Springer
… the complexity and dynamics of bacterial transcriptomes. The aim of … the use of RNAseq
results mapping of complete transcripts, … compared to the differential RNA-seq method include: …

Advances in bacterial transcriptome and transposon insertion-site profiling using second-generation sequencing

M Febrer, K McLay, M Caccamo, KB Twomey… - Trends in …, 2011 - cell.com
… The challenge facing bioinformaticians is how to process huge sequence data sets from
RNA-Seq experiments to (i) evaluate and describe transcript expression and (ii) reliably …

[PDF][PDF] Bacterial RNA biology on a genome scale

J Hör, SA Gorski, J Vogel - Molecular cell, 2018 - cell.com
… of a bacterium of interest. We review such approaches with a focus on RNA-seq as a versatile
… Top right: inhibition of Rho followed by RNA-seq reveals transcripts whose transcription is …

RNA-Seq and Microarrays Analyses Reveal Global Differential Transcriptomes of Mesorhizobium huakuii 7653R between Bacteroids and Free-Living Cells

J Peng, B Hao, L Liu, S Wang, B Ma, Y Yang, F Xie… - PLoS …, 2014 - journals.plos.org
bacterial RNA from the mixtures of plant and bacterial RNA samples. Our subsequent use
of … limited dynamic range for the detection of transcript levels due to background, saturation, …

Phenotype and RNA-seq-Based transcriptome profiling of Staphylococcus aureus biofilms in response to tea tree oil

X Zhao, Z Liu, Z Liu, R Meng, C Shi, X Chen, X Bu… - Microbial …, 2018 - Elsevier
… persistent and chronic bacterial infections [3]. It has been reported that biofilms supply … , the
transcript levels of the positive regulator, cidA, were decreased by 1.5-fold, and the transcript

RNA-seq reveals differentially expressed genes in rice (Oryza sativa) roots during interactions with plant-growth promoting bacteria, Azospirillum brasilense

J Thomas, HR Kim, Y Rahmatallah, G Wiggins… - PLoS …, 2019 - journals.plos.org
… identified in our RNA-Seq dataset, we performed reverse transcription polymerase chain …
confirm the expression pattern of these genes identified in the RNA-seq experiment (Fig 4). …