[PDF][PDF] Bacillus pasteurii urease: a heteropolymeric enzyme with a binuclear nickel active site
The purification of urease from Bacillus pasfeurii, a highly ureolytic soil bacterium (Larson
and Kallio, 1954), is reported. The enzyme is heteropolymeric, with a subunit composition of
a&y4 or a&.+. These findings, together with nickel analysis and inhibition studies, suggest
that B. pasteurii urease contains a binuclear nickel active site. Bacillus pasteurii ICDSM, type
strain 33), was grown aerobically at pH 9 and 28 C in a medium containing yeast extract (20
gL-'), urea (20 gL-'), and 1 zyxwvutsrqponmlkjihgfedcbaZYXWVUTSR… mM NiCl,, giving a …
and Kallio, 1954), is reported. The enzyme is heteropolymeric, with a subunit composition of
a&y4 or a&.+. These findings, together with nickel analysis and inhibition studies, suggest
that B. pasteurii urease contains a binuclear nickel active site. Bacillus pasteurii ICDSM, type
strain 33), was grown aerobically at pH 9 and 28 C in a medium containing yeast extract (20
gL-'), urea (20 gL-'), and 1 zyxwvutsrqponmlkjihgfedcbaZYXWVUTSR… mM NiCl,, giving a …
The purification of urease from Bacillus pasfeurii, a highly ureolytic soil bacterium (Larson and Kallio, 1954), is reported. The enzyme is heteropolymeric, with a subunit composition of a&y4 or a&.+. These findings, together with nickel analysis and inhibition studies, suggest that B. pasteurii urease contains a binuclear nickel active site.
Bacillus pasteurii ICDSM, type strain 33), was grown aerobically at pH 9 and 28 C in a medium containing yeast extract (20 gL-‘), urea (20 gL-‘), and 1 zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA mM NiCl,, giving a yield of 5 g of wet cells per liter of culture medium. Cells
academia.edu
以上显示的是最相近的搜索结果。 查看全部搜索结果